Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23330 | 5' | -54.8 | NC_005259.1 | + | 6619 | 1.11 | 0.001144 |
Target: 5'- uCGAUCAAGAUUGCCAGCGGGUCCGCCc -3' miRNA: 3'- -GCUAGUUCUAACGGUCGCCCAGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 9983 | 0.78 | 0.215758 |
Target: 5'- cCGGUCGAGGUgGCCAGcCGGGgacugaUCCGCg -3' miRNA: 3'- -GCUAGUUCUAaCGGUC-GCCC------AGGCGg -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 58283 | 0.77 | 0.251729 |
Target: 5'- aGAUCAGGuucgcgagGCCAGCGGauaCCGCCa -3' miRNA: 3'- gCUAGUUCuaa-----CGGUCGCCca-GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 66491 | 0.75 | 0.299773 |
Target: 5'- uCGAUCAugGGAUuuccuuccUGUCGGCGGGgcggucCCGCCu -3' miRNA: 3'- -GCUAGU--UCUA--------ACGGUCGCCCa-----GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 46276 | 0.75 | 0.330272 |
Target: 5'- uGcgCGAGGUucUGCaaCAGCGGGgCCGCCg -3' miRNA: 3'- gCuaGUUCUA--ACG--GUCGCCCaGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 57691 | 0.74 | 0.343909 |
Target: 5'- cCGuggCcAGGUUGCCGggcuugcccgacucGCGGGUCUGCCa -3' miRNA: 3'- -GCua-GuUCUAACGGU--------------CGCCCAGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 58790 | 0.73 | 0.416146 |
Target: 5'- cCGGUCGAGGcggugaUUGCCucGGCGGGcUCgCGCUg -3' miRNA: 3'- -GCUAGUUCU------AACGG--UCGCCC-AG-GCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 23345 | 0.72 | 0.473793 |
Target: 5'- uCGGgcUCGAGGcUGCCuGCGaGG-CCGCCg -3' miRNA: 3'- -GCU--AGUUCUaACGGuCGC-CCaGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 8661 | 0.72 | 0.483791 |
Target: 5'- uGGUCGGGc-UGCCAcGCGGGcaacggggCCGCCu -3' miRNA: 3'- gCUAGUUCuaACGGU-CGCCCa-------GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 5059 | 0.7 | 0.556279 |
Target: 5'- aCGGUCGAGAUcGUgGGcCGGGUCgacggGCCg -3' miRNA: 3'- -GCUAGUUCUAaCGgUC-GCCCAGg----CGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 26017 | 0.7 | 0.577614 |
Target: 5'- ---gCGGGAUgGCCgcaAGCGGGUUgGCCa -3' miRNA: 3'- gcuaGUUCUAaCGG---UCGCCCAGgCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 48589 | 0.69 | 0.620741 |
Target: 5'- aCGAUUGAGcUUGUCGagcGCGGGcgcuaCCGCCg -3' miRNA: 3'- -GCUAGUUCuAACGGU---CGCCCa----GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 12539 | 0.68 | 0.674789 |
Target: 5'- cCGAgCGAGccccacggcAUUGCCGGgGuGGcCCGCCa -3' miRNA: 3'- -GCUaGUUC---------UAACGGUCgC-CCaGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 4222 | 0.68 | 0.706828 |
Target: 5'- aGAUCGAGcagGCCaAGCaGGccCCGCCc -3' miRNA: 3'- gCUAGUUCuaaCGG-UCGcCCa-GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 2133 | 0.68 | 0.716329 |
Target: 5'- aGGUCAcgcagacccgcacGGugcugGCCAGCGGcGUcgagCCGCCg -3' miRNA: 3'- gCUAGU-------------UCuaa--CGGUCGCC-CA----GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 6194 | 0.67 | 0.73823 |
Target: 5'- uGAUCuGAGcAgcGUgAGCGGGgugagCCGCCg -3' miRNA: 3'- gCUAG-UUC-UaaCGgUCGCCCa----GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 60574 | 0.67 | 0.747482 |
Target: 5'- aCGGUCAGGcg-GCCcacggcgAGuCGGGUgCCGUCa -3' miRNA: 3'- -GCUAGUUCuaaCGG-------UC-GCCCA-GGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 37282 | 0.67 | 0.748505 |
Target: 5'- cCGG-CAGGAcUUGCaUGGCGGuGcCCGCCg -3' miRNA: 3'- -GCUaGUUCU-AACG-GUCGCC-CaGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 19247 | 0.67 | 0.758665 |
Target: 5'- uGAUCGAuGGccGCCuGcCGGG-CCGCCc -3' miRNA: 3'- gCUAGUU-CUaaCGGuC-GCCCaGGCGG- -5' |
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23330 | 5' | -54.8 | NC_005259.1 | + | 28894 | 0.66 | 0.778599 |
Target: 5'- -cGUCAagcAGAUUGUCGGCGacgaGGaacgCCGCCu -3' miRNA: 3'- gcUAGU---UCUAACGGUCGC----CCa---GGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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