Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23330 | 5' | -54.8 | NC_005259.1 | + | 18932 | 0.66 | 0.788351 |
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Target: 5'- uCGAUCAuccuGAUcaagGCCAGCaacgaggaugGGGUgaccUCGCCg -3' miRNA: 3'- -GCUAGUu---CUAa---CGGUCG----------CCCA----GGCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 8758 | 0.66 | 0.797945 |
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Target: 5'- aGGUCGAGGcccuugGCCAGCGcGGcggugaugagcUCgGCCc -3' miRNA: 3'- gCUAGUUCUaa----CGGUCGC-CC-----------AGgCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 36350 | 0.66 | 0.823886 |
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Target: 5'- gCGAUCuguugcagcucaaGGGAUUGCgucgaagCGGCGaGGUUgGCCa -3' miRNA: 3'- -GCUAG-------------UUCUAACG-------GUCGC-CCAGgCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 18819 | 0.66 | 0.825682 |
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Target: 5'- aGAcCAAGGUgcuguaucUGcCCGGUgaGGGUCuCGCCg -3' miRNA: 3'- gCUaGUUCUA--------AC-GGUCG--CCCAG-GCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 38498 | 0.66 | 0.825682 |
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Target: 5'- aGAUCGGuc-UG-UAGCGGG-CCGCCg -3' miRNA: 3'- gCUAGUUcuaACgGUCGCCCaGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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