miRNA display CGI


Results 21 - 25 of 25 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23330 5' -54.8 NC_005259.1 + 57691 0.74 0.343909
Target:  5'- cCGuggCcAGGUUGCCGggcuugcccgacucGCGGGUCUGCCa -3'
miRNA:   3'- -GCua-GuUCUAACGGU--------------CGCCCAGGCGG- -5'
23330 5' -54.8 NC_005259.1 + 46276 0.75 0.330272
Target:  5'- uGcgCGAGGUucUGCaaCAGCGGGgCCGCCg -3'
miRNA:   3'- gCuaGUUCUA--ACG--GUCGCCCaGGCGG- -5'
23330 5' -54.8 NC_005259.1 + 66491 0.75 0.299773
Target:  5'- uCGAUCAugGGAUuuccuuccUGUCGGCGGGgcggucCCGCCu -3'
miRNA:   3'- -GCUAGU--UCUA--------ACGGUCGCCCa-----GGCGG- -5'
23330 5' -54.8 NC_005259.1 + 58283 0.77 0.251729
Target:  5'- aGAUCAGGuucgcgagGCCAGCGGauaCCGCCa -3'
miRNA:   3'- gCUAGUUCuaa-----CGGUCGCCca-GGCGG- -5'
23330 5' -54.8 NC_005259.1 + 9983 0.78 0.215758
Target:  5'- cCGGUCGAGGUgGCCAGcCGGGgacugaUCCGCg -3'
miRNA:   3'- -GCUAGUUCUAaCGGUC-GCCC------AGGCGg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.