Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23339 | 5' | -61.3 | NC_005259.1 | + | 15742 | 0.66 | 0.49809 |
Target: 5'- ---aUUUCC-UCGCCGCCUacgagGCCGGg -3' miRNA: 3'- accaAAGGGcAGCGGCGGAg----CGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 50694 | 0.66 | 0.49809 |
Target: 5'- aGGcgcugCUCGUCGUCGCgCUUgaGCCGGg -3' miRNA: 3'- aCCaaa--GGGCAGCGGCG-GAG--CGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 31866 | 0.66 | 0.488372 |
Target: 5'- uUGGcgaUCuuGUCgGUCGugaCCUCGCCGGUc -3' miRNA: 3'- -ACCaa-AGggCAG-CGGC---GGAGCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 7309 | 0.66 | 0.488372 |
Target: 5'- uUGGgugCCCGaguUCaCCGCCgUCGCCGaGUa -3' miRNA: 3'- -ACCaaaGGGC---AGcGGCGG-AGCGGC-CA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 50203 | 0.66 | 0.46921 |
Target: 5'- ----cUCCCGcucaagCGCCGCCUCGUCa-- -3' miRNA: 3'- accaaAGGGCa-----GCGGCGGAGCGGcca -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 31680 | 0.66 | 0.46921 |
Target: 5'- gGGUUgCCCGagGCauCCUCGgCGGUg -3' miRNA: 3'- aCCAAaGGGCagCGgcGGAGCgGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 48177 | 0.66 | 0.459774 |
Target: 5'- -----cCCCGcCGCCGCCgggUGUCGGa -3' miRNA: 3'- accaaaGGGCaGCGGCGGa--GCGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 45334 | 0.66 | 0.459774 |
Target: 5'- cUGcGUggCCUG-CGCCGCCUgggcCGCCGcGUa -3' miRNA: 3'- -AC-CAaaGGGCaGCGGCGGA----GCGGC-CA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 20158 | 0.66 | 0.458836 |
Target: 5'- cGGUgcCCUGgccaccgcgccCGCCGCCucggcugacgagaUCGCCGGUu -3' miRNA: 3'- aCCAaaGGGCa----------GCGGCGG-------------AGCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 42211 | 0.66 | 0.45044 |
Target: 5'- aUGGaugCCCGccgCGCCGCCgUCcCCGGc -3' miRNA: 3'- -ACCaaaGGGCa--GCGGCGG-AGcGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 31732 | 0.66 | 0.45044 |
Target: 5'- aGGUga-CCG-CGCCuGCCgcgagCGCUGGUg -3' miRNA: 3'- aCCAaagGGCaGCGG-CGGa----GCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 56221 | 0.67 | 0.441211 |
Target: 5'- cGGUguaugCCaguCGUCGCCGCCgaccaCGuuGGg -3' miRNA: 3'- aCCAaa---GG---GCAGCGGCGGa----GCggCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 33206 | 0.67 | 0.43209 |
Target: 5'- aUGGaucagcacgUUCuuGUUGCCGCC--GCCGGUc -3' miRNA: 3'- -ACCa--------AAGggCAGCGGCGGagCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 25909 | 0.67 | 0.42308 |
Target: 5'- aUGGccgCCUGa-GCCGCCgcCGCCGGUc -3' miRNA: 3'- -ACCaaaGGGCagCGGCGGa-GCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 58860 | 0.67 | 0.405404 |
Target: 5'- cGGgcUCgCCGUCGUCGUCgUCGCCu-- -3' miRNA: 3'- aCCaaAG-GGCAGCGGCGG-AGCGGcca -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 36874 | 0.68 | 0.37978 |
Target: 5'- cUGGcaccgCCCG-CGCCGaCgaCGCCGGUg -3' miRNA: 3'- -ACCaaa--GGGCaGCGGC-GgaGCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 36666 | 0.68 | 0.37978 |
Target: 5'- -----gCCC-UCGCCGCCgcCGCCGGg -3' miRNA: 3'- accaaaGGGcAGCGGCGGa-GCGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 45494 | 0.69 | 0.331882 |
Target: 5'- cUGGUggCCUugGUCGCUgugGCCaccgCGCCGGUg -3' miRNA: 3'- -ACCAaaGGG--CAGCGG---CGGa---GCGGCCA- -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 53715 | 0.69 | 0.324344 |
Target: 5'- gUGGUca-CCG-CGCCGCUgauggugaugUCGCCGGg -3' miRNA: 3'- -ACCAaagGGCaGCGGCGG----------AGCGGCCa -5' |
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23339 | 5' | -61.3 | NC_005259.1 | + | 64191 | 0.69 | 0.309652 |
Target: 5'- cGGgaucUUCCuCGaUGUCGaCCUCGCCGGUc -3' miRNA: 3'- aCCa---AAGG-GCaGCGGC-GGAGCGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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