Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23343 | 5' | -57.9 | NC_005259.1 | + | 68797 | 0.69 | 0.464526 |
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Target: 5'- cGCCGGGG-CGgUGUCGgUGCccGGUACa -3' miRNA: 3'- aCGGCUCCaGCgGCAGC-ACG--UCAUGc -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 50405 | 0.69 | 0.4521 |
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Target: 5'- cGCCGAGcG-CGCCGgugccuugaccggcUCGggcaGCGGUGCGg -3' miRNA: 3'- aCGGCUC-CaGCGGC--------------AGCa---CGUCAUGC- -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 53609 | 0.7 | 0.399879 |
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Target: 5'- cGUCGGGGUCaGCaggCGUCGaGCGGUGCc -3' miRNA: 3'- aCGGCUCCAG-CG---GCAGCaCGUCAUGc -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 55039 | 0.71 | 0.357366 |
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Target: 5'- cUGCCGAGGUgcuUGCC-UCGgugGCAGUcuGCGc -3' miRNA: 3'- -ACGGCUCCA---GCGGcAGCa--CGUCA--UGC- -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 13420 | 0.72 | 0.318126 |
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Target: 5'- gGCCGGGGUCGCCGcCGggGCcGcGCu -3' miRNA: 3'- aCGGCUCCAGCGGCaGCa-CGuCaUGc -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 57970 | 0.72 | 0.310677 |
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Target: 5'- gGUCGAGGUCGCCGggaUCGUGacccCGGU-CGa -3' miRNA: 3'- aCGGCUCCAGCGGC---AGCAC----GUCAuGC- -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 48186 | 0.75 | 0.198772 |
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Target: 5'- cGCCGGGuGUCggaccgggcauGCCGUCGUaGUGGUACGa -3' miRNA: 3'- aCGGCUC-CAG-----------CGGCAGCA-CGUCAUGC- -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 33051 | 0.76 | 0.188778 |
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Target: 5'- uUGCCGGGGUCGCuCG-CGUGCuucuGGUAUc -3' miRNA: 3'- -ACGGCUCCAGCG-GCaGCACG----UCAUGc -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 35278 | 0.76 | 0.18829 |
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Target: 5'- cGCCGGGGUCGCCGaUGUGCcccuugaAGUAg- -3' miRNA: 3'- aCGGCUCCAGCGGCaGCACG-------UCAUgc -5' |
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| 23343 | 5' | -57.9 | NC_005259.1 | + | 2742 | 1.09 | 0.000786 |
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Target: 5'- aUGCCGAGGUCGCCGUCGUGCAGUACGa -3' miRNA: 3'- -ACGGCUCCAGCGGCAGCACGUCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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