Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23344 | 3' | -55.8 | NC_005259.1 | + | 54675 | 0.66 | 0.762241 |
Target: 5'- gCGUaUGCUCGGCgaGCGAGACCguguCGAGgCGg -3' miRNA: 3'- -GCA-GUGAGUCG--UGCUCUGG----GUUCgGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 20139 | 0.66 | 0.762241 |
Target: 5'- cCGUCGCgaugacggCAGC-CGGuGCCCuGGCCa -3' miRNA: 3'- -GCAGUGa-------GUCGuGCUcUGGGuUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 52883 | 0.66 | 0.742005 |
Target: 5'- aGUCACUCGggcGCGCGAGuacgggcaccuGCUCAcgaccGCCGa -3' miRNA: 3'- gCAGUGAGU---CGUGCUC-----------UGGGUu----CGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 6555 | 0.66 | 0.731722 |
Target: 5'- gGUgaaGCUguGCGCGGcGugUCAGGCCGc -3' miRNA: 3'- gCAg--UGAguCGUGCU-CugGGUUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 47898 | 0.67 | 0.725506 |
Target: 5'- gCGUCgGCUCguugAGCGCcuugauggcgucgccGAGGCCCuggAAGCCGa -3' miRNA: 3'- -GCAG-UGAG----UCGUG---------------CUCUGGG---UUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 67594 | 0.67 | 0.689743 |
Target: 5'- gCGUCG---GGCGCGAGACguguUCGAGCCa -3' miRNA: 3'- -GCAGUgagUCGUGCUCUG----GGUUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 63378 | 0.67 | 0.668393 |
Target: 5'- aCGUC-CUCGucGC-CGAuGGCCCAcGCCGa -3' miRNA: 3'- -GCAGuGAGU--CGuGCU-CUGGGUuCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 63435 | 0.68 | 0.657664 |
Target: 5'- aGcCGCUCgGGCGagacauCGAGGCCCAuGGCCa -3' miRNA: 3'- gCaGUGAG-UCGU------GCUCUGGGU-UCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 39341 | 0.68 | 0.657664 |
Target: 5'- cCGUCaACUCGacCACGAcGugCCGGGCCu -3' miRNA: 3'- -GCAG-UGAGUc-GUGCU-CugGGUUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 13514 | 0.68 | 0.636148 |
Target: 5'- gGUCugUCGGuCAcCGAGACUgcGGCCa -3' miRNA: 3'- gCAGugAGUC-GU-GCUCUGGguUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 55022 | 0.68 | 0.614618 |
Target: 5'- gGUCGuCUCGGCACGGGcuGCCgAGGUg- -3' miRNA: 3'- gCAGU-GAGUCGUGCUC--UGGgUUCGgc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 61086 | 0.69 | 0.597432 |
Target: 5'- gCGUCG-UCGGCcuCGGGcuuguugagccaguaGCCCGAGCCGa -3' miRNA: 3'- -GCAGUgAGUCGu-GCUC---------------UGGGUUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 56858 | 0.69 | 0.582453 |
Target: 5'- uGUUGgUCAGUGCGAGcacACCCucgauGAGCCGa -3' miRNA: 3'- gCAGUgAGUCGUGCUC---UGGG-----UUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 61936 | 0.69 | 0.561193 |
Target: 5'- gGUCuCUC-GCGCGGGugCCGGGgCCa -3' miRNA: 3'- gCAGuGAGuCGUGCUCugGGUUC-GGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 2749 | 0.69 | 0.561193 |
Target: 5'- gGUCGCcgucgugCAGUACGAccaccucCCCGAGCCGa -3' miRNA: 3'- gCAGUGa------GUCGUGCUcu-----GGGUUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 2941 | 0.7 | 0.509116 |
Target: 5'- gCGUCcaaggcGCGCGAGACCCuGGCCa -3' miRNA: 3'- -GCAGugagu-CGUGCUCUGGGuUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 42921 | 0.7 | 0.498936 |
Target: 5'- gGUgGCcuUgAGCAUGAGGCCCucGCCGu -3' miRNA: 3'- gCAgUG--AgUCGUGCUCUGGGuuCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 4175 | 0.71 | 0.449505 |
Target: 5'- gGUCACgcugCuGCGCGAGACCaaccgCAAGCuCGa -3' miRNA: 3'- gCAGUGa---GuCGUGCUCUGG-----GUUCG-GC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 39478 | 0.72 | 0.430499 |
Target: 5'- uCGUCGCggUAGCACGAGAUaUCGAGCUu -3' miRNA: 3'- -GCAGUGa-GUCGUGCUCUG-GGUUCGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 62863 | 0.72 | 0.421174 |
Target: 5'- aGcCGCUCGGCGCGAGG--UAGGCCGc -3' miRNA: 3'- gCaGUGAGUCGUGCUCUggGUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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