Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23346 | 3' | -55.8 | NC_005259.1 | + | 55362 | 0.69 | 0.607485 |
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Target: 5'- -cGCCUGAGCgACGGCAgcCGUgaucaGUGCc -3' miRNA: 3'- gcUGGGCUCG-UGCCGUauGCAg----CACG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 49606 | 0.7 | 0.569348 |
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Target: 5'- uCGACCuCGGGCAgcucaucaucgucauCGGCGgcagccGCGUCG-GCg -3' miRNA: 3'- -GCUGG-GCUCGU---------------GCCGUa-----UGCAGCaCG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 23677 | 0.7 | 0.560946 |
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Target: 5'- cCGAcCCCGAGCA-GGCGcacgcagacuaccGCGUCGUGa -3' miRNA: 3'- -GCU-GGGCUCGUgCCGUa------------UGCAGCACg -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 66114 | 0.7 | 0.554666 |
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Target: 5'- gCGACUucuacgcgcguCGAGCaggccaucagACGGCGUGCGUUG-GCa -3' miRNA: 3'- -GCUGG-----------GCUCG----------UGCCGUAUGCAGCaCG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 1412 | 1.12 | 0.000861 |
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Target: 5'- aCGACCCGAGCACGGCAUACGUCGUGCu -3' miRNA: 3'- -GCUGGGCUCGUGCCGUAUGCAGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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