Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23346 | 3' | -55.8 | NC_005259.1 | + | 61066 | 0.79 | 0.168297 |
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Target: 5'- uGugCCGGGCAuCGGCAUagGCGUCGUcgGCc -3' miRNA: 3'- gCugGGCUCGU-GCCGUA--UGCAGCA--CG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 61120 | 0.72 | 0.454069 |
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Target: 5'- cCGAgCCGAGCACGGUgc-CGUucucgaucCGUGCc -3' miRNA: 3'- -GCUgGGCUCGUGCCGuauGCA--------GCACG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 61248 | 0.67 | 0.703032 |
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Target: 5'- uCGAUCuCGGGUGCGaGCAUGCGacCGUcGCu -3' miRNA: 3'- -GCUGG-GCUCGUGC-CGUAUGCa-GCA-CG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 65098 | 0.7 | 0.513403 |
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Target: 5'- uCGGCUCGuGGCACGGUcaGUGCGUCaccgucgGCg -3' miRNA: 3'- -GCUGGGC-UCGUGCCG--UAUGCAGca-----CG- -5' |
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| 23346 | 3' | -55.8 | NC_005259.1 | + | 66114 | 0.7 | 0.554666 |
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Target: 5'- gCGACUucuacgcgcguCGAGCaggccaucagACGGCGUGCGUUG-GCa -3' miRNA: 3'- -GCUGG-----------GCUCG----------UGCCGUAUGCAGCaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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