Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23349 | 3' | -55.4 | NC_005259.1 | + | 8383 | 0.68 | 0.651305 |
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Target: 5'- -cGGCAUCgGCGAGA---GCcUCGGCGa -3' miRNA: 3'- caCCGUAG-CGCUCUuagUGuGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 50967 | 0.68 | 0.650223 |
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Target: 5'- cGUGGCuGUCccacgcgGCGAGcgguGUCGaguCACCGGCGa -3' miRNA: 3'- -CACCG-UAG-------CGCUCu---UAGU---GUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 55058 | 0.68 | 0.618804 |
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Target: 5'- gGUGGCAgucugCGCGAGAu---CAgCGGCu -3' miRNA: 3'- -CACCGUa----GCGCUCUuaguGUgGCCGc -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 9616 | 0.68 | 0.618804 |
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Target: 5'- cGUGGCAUCGaacac--CGCGCCGGUGc -3' miRNA: 3'- -CACCGUAGCgcucuuaGUGUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 12197 | 0.69 | 0.607981 |
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Target: 5'- cGUGGUcgUGUaGAGAu--GCACCGGCGc -3' miRNA: 3'- -CACCGuaGCG-CUCUuagUGUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 14157 | 0.7 | 0.522804 |
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Target: 5'- -aGcGCGUCGUGuGGAUCuCGCCgGGCGg -3' miRNA: 3'- caC-CGUAGCGCuCUUAGuGUGG-CCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 61604 | 0.72 | 0.433073 |
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Target: 5'- gGUGGgGUCgGCGGGGAacagCAgACCGGCa -3' miRNA: 3'- -CACCgUAG-CGCUCUUa---GUgUGGCCGc -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 42589 | 0.74 | 0.313208 |
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Target: 5'- -aGGCGUgaGCGAGcAGUCgACAUCGGCGg -3' miRNA: 3'- caCCGUAg-CGCUC-UUAG-UGUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 717 | 1.09 | 0.001238 |
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Target: 5'- gGUGGCAUCGCGAGAAUCACACCGGCGg -3' miRNA: 3'- -CACCGUAGCGCUCUUAGUGUGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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