Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23353 | 5' | -55.5 | NC_005259.1 | + | 46509 | 0.7 | 0.531987 |
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Target: 5'- -cUCGUCGuacGAUCCCGcgaGCCGGuCGg -3' miRNA: 3'- guAGUAGCu--CUAGGGCag-CGGCCuGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 18721 | 0.7 | 0.552751 |
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Target: 5'- gAUCAUCGGGccgCCCGgcaucgguaagUCGUCGGugGu -3' miRNA: 3'- gUAGUAGCUCua-GGGC-----------AGCGGCCugC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 46539 | 0.7 | 0.552751 |
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Target: 5'- gAUCGcCGAGAUCUgGUCGCCGaccGCGc -3' miRNA: 3'- gUAGUaGCUCUAGGgCAGCGGCc--UGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 61529 | 0.69 | 0.60557 |
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Target: 5'- -cUCGUCGGGAauuUCCuCGUC-UCGGGCGa -3' miRNA: 3'- guAGUAGCUCU---AGG-GCAGcGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 49213 | 0.69 | 0.61623 |
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Target: 5'- cCGUUGUgCGGGGUuuaCUCGUCGUCGGACu -3' miRNA: 3'- -GUAGUA-GCUCUA---GGGCAGCGGCCUGc -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 26389 | 0.69 | 0.626903 |
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Target: 5'- uGUUGcUCGcGAUUCCGaCGCCGGGCGc -3' miRNA: 3'- gUAGU-AGCuCUAGGGCaGCGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 66248 | 0.69 | 0.635446 |
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Target: 5'- --cCAUCGAcgcagcgcggCCCG-CGCCGGGCGg -3' miRNA: 3'- guaGUAGCUcua-------GGGCaGCGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 1541 | 0.66 | 0.810321 |
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Target: 5'- gCAUCAUCGGGG-CCgCGUU-CgGGGCGg -3' miRNA: 3'- -GUAGUAGCUCUaGG-GCAGcGgCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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