Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23353 | 5' | -55.5 | NC_005259.1 | + | 49706 | 0.66 | 0.800149 |
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Target: 5'- uGUCggUGAGAUCCgGcagauauUCGgCGGGCGg -3' miRNA: 3'- gUAGuaGCUCUAGGgC-------AGCgGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 57226 | 0.67 | 0.752591 |
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Target: 5'- gGUUGUCGGGGUCggagauccacgUCGUUGCCGGGa- -3' miRNA: 3'- gUAGUAGCUCUAG-----------GGCAGCGGCCUgc -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 58914 | 0.71 | 0.481437 |
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Target: 5'- -cUCGUCGGcGGUCuuGUCGUCGG-CGg -3' miRNA: 3'- guAGUAGCU-CUAGggCAGCGGCCuGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 60351 | 0.74 | 0.362556 |
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Target: 5'- gAUCAggUCGAGGcccugcccgaaaUCCCGccaaUCGCCGGACa -3' miRNA: 3'- gUAGU--AGCUCU------------AGGGC----AGCGGCCUGc -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 61529 | 0.69 | 0.60557 |
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Target: 5'- -cUCGUCGGGAauuUCCuCGUC-UCGGGCGa -3' miRNA: 3'- guAGUAGCUCU---AGG-GCAGcGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 62715 | 0.66 | 0.782115 |
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Target: 5'- gAUCAggcggGGGAUCUCGcUCGaCCGGACc -3' miRNA: 3'- gUAGUag---CUCUAGGGC-AGC-GGCCUGc -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 66248 | 0.69 | 0.635446 |
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Target: 5'- --cCAUCGAcgcagcgcggCCCG-CGCCGGGCGg -3' miRNA: 3'- guaGUAGCUcua-------GGGCaGCGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 68856 | 0.72 | 0.424049 |
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Target: 5'- cCAUCAugUCGAcuugCCCG-CGCCGGGCGc -3' miRNA: 3'- -GUAGU--AGCUcua-GGGCaGCGGCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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