Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23353 | 5' | -55.5 | NC_005259.1 | + | 16418 | 0.66 | 0.801082 |
Target: 5'- --cCAcCGAGGUgCCCGUCGagcggcccaaaCCGGGCa -3' miRNA: 3'- guaGUaGCUCUA-GGGCAGC-----------GGCCUGc -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 13671 | 0.66 | 0.782115 |
Target: 5'- aCAUCGacggugugcUCGuGGaCCCGUgGCCGGuCGa -3' miRNA: 3'- -GUAGU---------AGCuCUaGGGCAgCGGCCuGC- -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 10291 | 0.67 | 0.761571 |
Target: 5'- uCGUCAUCGAGGUUggaccaaUCGUCGUagucgucauCGGGCu -3' miRNA: 3'- -GUAGUAGCUCUAG-------GGCAGCG---------GCCUGc -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 8385 | 0.66 | 0.791677 |
Target: 5'- gCAUCggCGAGAgCCuCGgcgaGCCGGGCc -3' miRNA: 3'- -GUAGuaGCUCUaGG-GCag--CGGCCUGc -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 8073 | 0.67 | 0.752591 |
Target: 5'- --aCAUCGAGAUCgugCCG-CGCUGG-CGu -3' miRNA: 3'- guaGUAGCUCUAG---GGCaGCGGCCuGC- -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 7052 | 0.76 | 0.259126 |
Target: 5'- uCGUUgcgCGAGAUCCUGUCGaCGGGCGg -3' miRNA: 3'- -GUAGua-GCUCUAGGGCAGCgGCCUGC- -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 3264 | 0.68 | 0.68438 |
Target: 5'- gCAUCcugcUCGGGugguuUCCCGUCGCCGccucgccggucccucGGCGa -3' miRNA: 3'- -GUAGu---AGCUCu----AGGGCAGCGGC---------------CUGC- -5' |
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23353 | 5' | -55.5 | NC_005259.1 | + | 1541 | 0.66 | 0.810321 |
Target: 5'- gCAUCAUCGGGG-CCgCGUU-CgGGGCGg -3' miRNA: 3'- -GUAGUAGCUCUaGG-GCAGcGgCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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