Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23368 | 3' | -57.4 | NC_005259.1 | + | 9546 | 0.66 | 0.668393 |
Target: 5'- -cGGAgAGCuGCc-CGGCCUCgACCGCg -3' miRNA: 3'- cuCCUgUCGcCGauGCUGGAG-UGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 16704 | 0.66 | 0.667322 |
Target: 5'- cGAGGugAccgccgaGCaGGUcGCGcucuGCCUCGCCACg -3' miRNA: 3'- -CUCCugU-------CG-CCGaUGC----UGGAGUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 61606 | 0.66 | 0.657664 |
Target: 5'- uGGGGuCGGCGGggAacagcaGACCggCACCGCc -3' miRNA: 3'- -CUCCuGUCGCCgaUg-----CUGGa-GUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 8985 | 0.66 | 0.657664 |
Target: 5'- uAGGugcCAGCGGCgACGGCa--GCCGCc -3' miRNA: 3'- cUCCu--GUCGCCGaUGCUGgagUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 10184 | 0.66 | 0.633994 |
Target: 5'- uGAGGGCAGCacaucGUgaugACGACCgggcagcucgacCACCACg -3' miRNA: 3'- -CUCCUGUCGc----CGa---UGCUGGa-----------GUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 46086 | 0.66 | 0.62538 |
Target: 5'- cGGGGCgAGCGGU----GCCUCaACCACg -3' miRNA: 3'- cUCCUG-UCGCCGaugcUGGAG-UGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 50511 | 0.67 | 0.593146 |
Target: 5'- -uGGGCGGUGcGCUGC-ACCUCGaCGCg -3' miRNA: 3'- cuCCUGUCGC-CGAUGcUGGAGUgGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 53136 | 0.67 | 0.561193 |
Target: 5'- aGAGGAagaaaugaccauCAGCcGCUAcgccgaggcCGAUCUCGCCACc -3' miRNA: 3'- -CUCCU------------GUCGcCGAU---------GCUGGAGUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 18379 | 0.67 | 0.550642 |
Target: 5'- cGAGGGCGGUaGCgagggagACGAgcaCCUCGCCGa -3' miRNA: 3'- -CUCCUGUCGcCGa------UGCU---GGAGUGGUg -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 60389 | 0.67 | 0.550642 |
Target: 5'- -cGGACAGCGGCgagGCGagGCC-CAUCuCg -3' miRNA: 3'- cuCCUGUCGCCGa--UGC--UGGaGUGGuG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 32530 | 0.68 | 0.528692 |
Target: 5'- -cGGGCGGCaGGCU-UGACCUUgcuguugGCCGCc -3' miRNA: 3'- cuCCUGUCG-CCGAuGCUGGAG-------UGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 63091 | 0.68 | 0.519383 |
Target: 5'- uGAcGACAuCGGCUGCGAaCUCACgGCa -3' miRNA: 3'- -CUcCUGUcGCCGAUGCUgGAGUGgUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 13110 | 0.68 | 0.519383 |
Target: 5'- -cGGcccgcuCGGCGGCUACGACCa-GCgCACg -3' miRNA: 3'- cuCCu-----GUCGCCGAUGCUGGagUG-GUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 26530 | 0.68 | 0.509116 |
Target: 5'- -cGGugAGCaGCUuggccaccACGAUCUCACCGa -3' miRNA: 3'- cuCCugUCGcCGA--------UGCUGGAGUGGUg -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 46667 | 0.68 | 0.498936 |
Target: 5'- uGAGGuucuCGGCGaGCU-CGGCCUgagcCGCCGCc -3' miRNA: 3'- -CUCCu---GUCGC-CGAuGCUGGA----GUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 5726 | 0.68 | 0.498936 |
Target: 5'- -cGGACGGCGGCcauucucGCGGCCaaGCgGCg -3' miRNA: 3'- cuCCUGUCGCCGa------UGCUGGagUGgUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 3335 | 0.68 | 0.498936 |
Target: 5'- -cGGGCAGCu-CUGCGugCUCGgCACg -3' miRNA: 3'- cuCCUGUCGccGAUGCugGAGUgGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 37187 | 0.68 | 0.488848 |
Target: 5'- cGAGGACGa--GCUGCGGCCcUUGCCGCc -3' miRNA: 3'- -CUCCUGUcgcCGAUGCUGG-AGUGGUG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 5650 | 0.69 | 0.478856 |
Target: 5'- cGAGGACGGCgGGCgcuacacgcGCGACaacaUCGCCccGCa -3' miRNA: 3'- -CUCCUGUCG-CCGa--------UGCUGg---AGUGG--UG- -5' |
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23368 | 3' | -57.4 | NC_005259.1 | + | 48445 | 0.69 | 0.439943 |
Target: 5'- cGAGG-CAGCGGCaGCGcguUC-CACCGCg -3' miRNA: 3'- -CUCCuGUCGCCGaUGCu--GGaGUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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