Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23368 | 3' | -57.4 | NC_005259.1 | + | 1234 | 0.69 | 0.439943 |
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Target: 5'- cGAGGACGGCuGCaagacCGGCCUCGgCAUc -3' miRNA: 3'- -CUCCUGUCGcCGau---GCUGGAGUgGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 13213 | 0.7 | 0.430499 |
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Target: 5'- cGGGGcCAGCGGUgggccuCGACCg-GCCACc -3' miRNA: 3'- -CUCCuGUCGCCGau----GCUGGagUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 42060 | 0.7 | 0.385128 |
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Target: 5'- cGGGGugGGCGGCUAgGuAUCgcagCACCGa -3' miRNA: 3'- -CUCCugUCGCCGAUgC-UGGa---GUGGUg -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 47946 | 0.71 | 0.367886 |
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Target: 5'- cGAuGACGGCGGUgccgAUGGCCgccgCGCCGCc -3' miRNA: 3'- -CUcCUGUCGCCGa---UGCUGGa---GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 18958 | 0.71 | 0.359467 |
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Target: 5'- cGAGGAUGG-GGU---GACCUCGCCGCg -3' miRNA: 3'- -CUCCUGUCgCCGaugCUGGAGUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 66272 | 0.72 | 0.319417 |
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Target: 5'- -cGGGCGGCGGCUcACG-CCgcCACCAUc -3' miRNA: 3'- cuCCUGUCGCCGA-UGCuGGa-GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 4265 | 0.73 | 0.255977 |
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Target: 5'- cGAGGACAacggGCGGCUGCG-CU--GCCACg -3' miRNA: 3'- -CUCCUGU----CGCCGAUGCuGGagUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 31978 | 0.75 | 0.203241 |
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Target: 5'- cAGGACAGCGGUgACGGCCaUAgCACa -3' miRNA: 3'- cUCCUGUCGCCGaUGCUGGaGUgGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 62565 | 0.8 | 0.087613 |
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Target: 5'- -cGGGCAGCGGCcacaucagACGACCUCGCCc- -3' miRNA: 3'- cuCCUGUCGCCGa-------UGCUGGAGUGGug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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