Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23368 | 3' | -57.4 | NC_005259.1 | + | 47946 | 0.71 | 0.367886 |
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Target: 5'- cGAuGACGGCGGUgccgAUGGCCgccgCGCCGCc -3' miRNA: 3'- -CUcCUGUCGCCGa---UGCUGGa---GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 48445 | 0.69 | 0.439943 |
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Target: 5'- cGAGG-CAGCGGCaGCGcguUC-CACCGCg -3' miRNA: 3'- -CUCCuGUCGCCGaUGCu--GGaGUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 50511 | 0.67 | 0.593146 |
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Target: 5'- -uGGGCGGUGcGCUGC-ACCUCGaCGCg -3' miRNA: 3'- cuCCUGUCGC-CGAUGcUGGAGUgGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 53136 | 0.67 | 0.561193 |
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Target: 5'- aGAGGAagaaaugaccauCAGCcGCUAcgccgaggcCGAUCUCGCCACc -3' miRNA: 3'- -CUCCU------------GUCGcCGAU---------GCUGGAGUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 60389 | 0.67 | 0.550642 |
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Target: 5'- -cGGACAGCGGCgagGCGagGCC-CAUCuCg -3' miRNA: 3'- cuCCUGUCGCCGa--UGC--UGGaGUGGuG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 61606 | 0.66 | 0.657664 |
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Target: 5'- uGGGGuCGGCGGggAacagcaGACCggCACCGCc -3' miRNA: 3'- -CUCCuGUCGCCgaUg-----CUGGa-GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 62565 | 0.8 | 0.087613 |
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Target: 5'- -cGGGCAGCGGCcacaucagACGACCUCGCCc- -3' miRNA: 3'- cuCCUGUCGCCGa-------UGCUGGAGUGGug -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 63091 | 0.68 | 0.519383 |
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Target: 5'- uGAcGACAuCGGCUGCGAaCUCACgGCa -3' miRNA: 3'- -CUcCUGUcGCCGAUGCUgGAGUGgUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 66272 | 0.72 | 0.319417 |
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Target: 5'- -cGGGCGGCGGCUcACG-CCgcCACCAUc -3' miRNA: 3'- cuCCUGUCGCCGA-UGCuGGa-GUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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