Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23368 | 3' | -57.4 | NC_005259.1 | + | 18379 | 0.67 | 0.550642 |
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Target: 5'- cGAGGGCGGUaGCgagggagACGAgcaCCUCGCCGa -3' miRNA: 3'- -CUCCUGUCGcCGa------UGCU---GGAGUGGUg -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 53136 | 0.67 | 0.561193 |
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Target: 5'- aGAGGAagaaaugaccauCAGCcGCUAcgccgaggcCGAUCUCGCCACc -3' miRNA: 3'- -CUCCU------------GUCGcCGAU---------GCUGGAGUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 50511 | 0.67 | 0.593146 |
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Target: 5'- -uGGGCGGUGcGCUGC-ACCUCGaCGCg -3' miRNA: 3'- cuCCUGUCGC-CGAUGcUGGAGUgGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 46086 | 0.66 | 0.62538 |
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Target: 5'- cGGGGCgAGCGGU----GCCUCaACCACg -3' miRNA: 3'- cUCCUG-UCGCCGaugcUGGAG-UGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 10184 | 0.66 | 0.633994 |
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Target: 5'- uGAGGGCAGCacaucGUgaugACGACCgggcagcucgacCACCACg -3' miRNA: 3'- -CUCCUGUCGc----CGa---UGCUGGa-----------GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 8985 | 0.66 | 0.657664 |
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Target: 5'- uAGGugcCAGCGGCgACGGCa--GCCGCc -3' miRNA: 3'- cUCCu--GUCGCCGaUGCUGgagUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 61606 | 0.66 | 0.657664 |
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Target: 5'- uGGGGuCGGCGGggAacagcaGACCggCACCGCc -3' miRNA: 3'- -CUCCuGUCGCCgaUg-----CUGGa-GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 16704 | 0.66 | 0.667322 |
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Target: 5'- cGAGGugAccgccgaGCaGGUcGCGcucuGCCUCGCCACg -3' miRNA: 3'- -CUCCugU-------CG-CCGaUGC----UGGAGUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 9546 | 0.66 | 0.668393 |
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Target: 5'- -cGGAgAGCuGCc-CGGCCUCgACCGCg -3' miRNA: 3'- cuCCUgUCGcCGauGCUGGAG-UGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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