Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23368 | 3' | -57.4 | NC_005259.1 | + | 13110 | 0.68 | 0.519383 |
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Target: 5'- -cGGcccgcuCGGCGGCUACGACCa-GCgCACg -3' miRNA: 3'- cuCCu-----GUCGCCGAUGCUGGagUG-GUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 10184 | 0.66 | 0.633994 |
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Target: 5'- uGAGGGCAGCacaucGUgaugACGACCgggcagcucgacCACCACg -3' miRNA: 3'- -CUCCUGUCGc----CGa---UGCUGGa-----------GUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 9546 | 0.66 | 0.668393 |
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Target: 5'- -cGGAgAGCuGCc-CGGCCUCgACCGCg -3' miRNA: 3'- cuCCUgUCGcCGauGCUGGAG-UGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 8985 | 0.66 | 0.657664 |
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Target: 5'- uAGGugcCAGCGGCgACGGCa--GCCGCc -3' miRNA: 3'- cUCCu--GUCGCCGaUGCUGgagUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 5726 | 0.68 | 0.498936 |
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Target: 5'- -cGGACGGCGGCcauucucGCGGCCaaGCgGCg -3' miRNA: 3'- cuCCUGUCGCCGa------UGCUGGagUGgUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 5650 | 0.69 | 0.478856 |
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Target: 5'- cGAGGACGGCgGGCgcuacacgcGCGACaacaUCGCCccGCa -3' miRNA: 3'- -CUCCUGUCG-CCGa--------UGCUGg---AGUGG--UG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 4265 | 0.73 | 0.255977 |
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Target: 5'- cGAGGACAacggGCGGCUGCG-CU--GCCACg -3' miRNA: 3'- -CUCCUGU----CGCCGAUGCuGGagUGGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 3335 | 0.68 | 0.498936 |
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Target: 5'- -cGGGCAGCu-CUGCGugCUCGgCACg -3' miRNA: 3'- cuCCUGUCGccGAUGCugGAGUgGUG- -5' |
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| 23368 | 3' | -57.4 | NC_005259.1 | + | 1234 | 0.69 | 0.439943 |
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Target: 5'- cGAGGACGGCuGCaagacCGGCCUCGgCAUc -3' miRNA: 3'- -CUCCUGUCGcCGau---GCUGGAGUgGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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