Results 1 - 20 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23377 | 5' | -59.5 | NC_005259.1 | + | 63616 | 0.74 | 0.187038 |
Target: 5'- cGGCCACCGacGCCUGAuagaUCaucguggcgagcugcUCGCUGgCCGg -3' miRNA: 3'- -CCGGUGGC--UGGACU----AG---------------AGCGACgGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 1827 | 0.73 | 0.221123 |
Target: 5'- -cCCGCCGACCUGcUCgcCGCUGCCg- -3' miRNA: 3'- ccGGUGGCUGGACuAGa-GCGACGGgc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 22443 | 0.73 | 0.232451 |
Target: 5'- gGGCCAUCGACuCUGGcuacCUCGUgcGCCCGc -3' miRNA: 3'- -CCGGUGGCUG-GACUa---GAGCGa-CGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 30362 | 0.73 | 0.232451 |
Target: 5'- uGGaCGCCGAUCUGcc--CGCUGCCCGg -3' miRNA: 3'- -CCgGUGGCUGGACuagaGCGACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 53524 | 0.73 | 0.238296 |
Target: 5'- uGGCCAgCGGCacgGG-CUCGgUGCCCGg -3' miRNA: 3'- -CCGGUgGCUGga-CUaGAGCgACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46552 | 0.72 | 0.272664 |
Target: 5'- uGGUCGCCGACCgcgccuaUGGUCgcggcgucgaccgCGCcGCCCGa -3' miRNA: 3'- -CCGGUGGCUGG-------ACUAGa------------GCGaCGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 19059 | 0.72 | 0.275978 |
Target: 5'- cGCCACCGAUgUcGGUgUCGCgGUCCGg -3' miRNA: 3'- cCGGUGGCUGgA-CUAgAGCGaCGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 42955 | 0.71 | 0.280671 |
Target: 5'- aGGCCGCCGuugucgaacgggauGCCgggGAUCaUCGUUGCCg- -3' miRNA: 3'- -CCGGUGGC--------------UGGa--CUAG-AGCGACGGgc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 54758 | 0.71 | 0.289556 |
Target: 5'- aGGCCGCCGACCcaaacCUUGCuuaUGCCUGc -3' miRNA: 3'- -CCGGUGGCUGGacua-GAGCG---ACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 61956 | 0.71 | 0.289556 |
Target: 5'- gGGCCACCGcaGCCUcacGcgC-CGCUGCCuCGa -3' miRNA: 3'- -CCGGUGGC--UGGA---CuaGaGCGACGG-GC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 56114 | 0.71 | 0.29654 |
Target: 5'- uGGCCACCGAaaCUGugugCGCUGCCgGu -3' miRNA: 3'- -CCGGUGGCUg-GACuagaGCGACGGgC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 56238 | 0.71 | 0.304372 |
Target: 5'- cGCCGCCGACCacguuggGAUCggcggccuugagcgcCGCcGCCCGg -3' miRNA: 3'- cCGGUGGCUGGa------CUAGa--------------GCGaCGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 67772 | 0.71 | 0.310897 |
Target: 5'- cGGCCACCGACCacgcggUGAcCUUGUUGgCaCCGc -3' miRNA: 3'- -CCGGUGGCUGG------ACUaGAGCGAC-G-GGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 63679 | 0.7 | 0.325778 |
Target: 5'- cGGgCACCGgcgcGCCgggGAUCUgCGCcUGCCCa -3' miRNA: 3'- -CCgGUGGC----UGGa--CUAGA-GCG-ACGGGc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46948 | 0.7 | 0.333414 |
Target: 5'- cGCCGCCGACCgcguUGAgCUUGUUGgCCa -3' miRNA: 3'- cCGGUGGCUGG----ACUaGAGCGACgGGc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 49101 | 0.7 | 0.341181 |
Target: 5'- uGGCCGCCcGCCggGAUCUcaagaCGCUGaucaCCGg -3' miRNA: 3'- -CCGGUGGcUGGa-CUAGA-----GCGACg---GGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 9532 | 0.7 | 0.341181 |
Target: 5'- cGCCGCCGggucACCgGAga--GCUGCCCGg -3' miRNA: 3'- cCGGUGGC----UGGaCUagagCGACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 40689 | 0.7 | 0.349077 |
Target: 5'- uGCuCACCGGCCcgaUGAUCggGgUGCCCGc -3' miRNA: 3'- cCG-GUGGCUGG---ACUAGagCgACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 35709 | 0.7 | 0.352272 |
Target: 5'- gGGUCGCUGACgaGGUauccgcccgacgaccCggCGCUGCCCGg -3' miRNA: 3'- -CCGGUGGCUGgaCUA---------------Ga-GCGACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 33133 | 0.69 | 0.381949 |
Target: 5'- aGCCGCCGcGCgUGAUCUUGCcguagaccGCCUGa -3' miRNA: 3'- cCGGUGGC-UGgACUAGAGCGa-------CGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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