Results 21 - 40 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23377 | 5' | -59.5 | NC_005259.1 | + | 49101 | 0.7 | 0.341181 |
Target: 5'- uGGCCGCCcGCCggGAUCUcaagaCGCUGaucaCCGg -3' miRNA: 3'- -CCGGUGGcUGGa-CUAGA-----GCGACg---GGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46948 | 0.7 | 0.333414 |
Target: 5'- cGCCGCCGACCgcguUGAgCUUGUUGgCCa -3' miRNA: 3'- cCGGUGGCUGG----ACUaGAGCGACgGGc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46818 | 0.66 | 0.562524 |
Target: 5'- -aCCACCGgccACCUGAgugagCgccccggcgaGCUGCCCGa -3' miRNA: 3'- ccGGUGGC---UGGACUa----Gag--------CGACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46552 | 0.72 | 0.272664 |
Target: 5'- uGGUCGCCGACCgcgccuaUGGUCgcggcgucgaccgCGCcGCCCGa -3' miRNA: 3'- -CCGGUGGCUGG-------ACUAGa------------GCGaCGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 46298 | 0.66 | 0.542029 |
Target: 5'- gGGCCGCCGuGgCUGcguugUUCGCgGCCCc -3' miRNA: 3'- -CCGGUGGC-UgGACua---GAGCGaCGGGc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 45603 | 0.67 | 0.511777 |
Target: 5'- --gCACCGACagcucGGUCUCGaUGCCCGc -3' miRNA: 3'- ccgGUGGCUGga---CUAGAGCgACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 43966 | 0.68 | 0.434967 |
Target: 5'- gGGCCGCCG-CCUGAcCgagggCGgUGCCg- -3' miRNA: 3'- -CCGGUGGCuGGACUaGa----GCgACGGgc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 42955 | 0.71 | 0.280671 |
Target: 5'- aGGCCGCCGuugucgaacgggauGCCgggGAUCaUCGUUGCCg- -3' miRNA: 3'- -CCGGUGGC--------------UGGa--CUAG-AGCGACGGgc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 42663 | 0.66 | 0.572851 |
Target: 5'- cGGCCACgCGGCUgcuguuggcGAUCUCGac-CCCGu -3' miRNA: 3'- -CCGGUG-GCUGGa--------CUAGAGCgacGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 40689 | 0.7 | 0.349077 |
Target: 5'- uGCuCACCGGCCcgaUGAUCggGgUGCCCGc -3' miRNA: 3'- cCG-GUGGCUGG---ACUAGagCgACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 38799 | 0.69 | 0.416819 |
Target: 5'- cGCUGCCGAUgaGAUCgccCGC-GCCCGc -3' miRNA: 3'- cCGGUGGCUGgaCUAGa--GCGaCGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 38682 | 0.66 | 0.589463 |
Target: 5'- uGGCCGCCGggucgagguuggccACCUGAgaCUguagguugaccacCGCUGCCg- -3' miRNA: 3'- -CCGGUGGC--------------UGGACUa-GA-------------GCGACGGgc -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 37275 | 0.66 | 0.552248 |
Target: 5'- aGGCCGCCcggcagGACUUGcAUggCGgUGCCCGc -3' miRNA: 3'- -CCGGUGG------CUGGAC-UAgaGCgACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 37216 | 0.67 | 0.482247 |
Target: 5'- cGCCGCCGccGCCgGGg--CGCUgGCCCGu -3' miRNA: 3'- cCGGUGGC--UGGaCUagaGCGA-CGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 36782 | 0.68 | 0.434967 |
Target: 5'- uGCCGCCGuCCUGAccgCUCGCgucgGCauuguugaCCGg -3' miRNA: 3'- cCGGUGGCuGGACUa--GAGCGa---CG--------GGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 36671 | 0.66 | 0.59363 |
Target: 5'- cGCCGCCGccGCCggGGUCUUGaccguccucGCCCGc -3' miRNA: 3'- cCGGUGGC--UGGa-CUAGAGCga-------CGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 36135 | 0.68 | 0.444212 |
Target: 5'- gGGCCGCCGGgCUG-UCcgUCGCcaugGCCaCGg -3' miRNA: 3'- -CCGGUGGCUgGACuAG--AGCGa---CGG-GC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 35709 | 0.7 | 0.352272 |
Target: 5'- gGGUCGCUGACgaGGUauccgcccgacgaccCggCGCUGCCCGg -3' miRNA: 3'- -CCGGUGGCUGgaCUA---------------Ga-GCGACGGGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 35566 | 0.67 | 0.492002 |
Target: 5'- uGCCGCCGACCgccgaGAUCcCGUUGUagCUGa -3' miRNA: 3'- cCGGUGGCUGGa----CUAGaGCGACG--GGC- -5' |
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23377 | 5' | -59.5 | NC_005259.1 | + | 33623 | 0.66 | 0.59363 |
Target: 5'- cGGCCACCGAaaCUGuugcgaaUCGC-GCUCGg -3' miRNA: 3'- -CCGGUGGCUg-GACuag----AGCGaCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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