Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23378 | 3' | -57.8 | NC_005259.1 | + | 21928 | 0.66 | 0.607485 |
Target: 5'- aGGCagUCGAGGCCAUcGAgucUCaCUGGCGCa -3' miRNA: 3'- -UCG--AGCUCCGGUGaCU---GG-GACUGUGg -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 37225 | 0.66 | 0.639478 |
Target: 5'- cGC-CGGGG-CGCUGGCCCgugccggauUGGCuGCCg -3' miRNA: 3'- uCGaGCUCCgGUGACUGGG---------ACUG-UGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 50447 | 0.66 | 0.628808 |
Target: 5'- gGGCUCGGGGUCGCcgGucuCgCUGAuCACg -3' miRNA: 3'- -UCGAGCUCCGGUGa-Cu--GgGACU-GUGg -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 547 | 0.66 | 0.639478 |
Target: 5'- -uCUCGuGGaCCaaGCUGACCC--GCACCa -3' miRNA: 3'- ucGAGCuCC-GG--UGACUGGGacUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 17820 | 0.66 | 0.650141 |
Target: 5'- gAGCUcagCGAGGCCAUcgacgggugGGCCgaGGcCACCc -3' miRNA: 3'- -UCGA---GCUCCGGUGa--------CUGGgaCU-GUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 10341 | 0.66 | 0.650141 |
Target: 5'- gGGCUCGGcGGCaGCgGGCUC-GACAUCg -3' miRNA: 3'- -UCGAGCU-CCGgUGaCUGGGaCUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 15139 | 0.66 | 0.650141 |
Target: 5'- aGGUguaCGGGGCCACcucggUGGCCgaGGCcgagGCCa -3' miRNA: 3'- -UCGa--GCUCCGGUG-----ACUGGgaCUG----UGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 60421 | 0.66 | 0.628808 |
Target: 5'- uGCUCGGGGguggcCCACggggcGGCCaCgcccGACACCu -3' miRNA: 3'- uCGAGCUCC-----GGUGa----CUGG-Ga---CUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 21004 | 0.66 | 0.628808 |
Target: 5'- -aCUCG-GGCgGC-GACCCUGACcggcagcugACCg -3' miRNA: 3'- ucGAGCuCCGgUGaCUGGGACUG---------UGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 18177 | 0.66 | 0.628808 |
Target: 5'- uGGCagUCGAGGUC---GACCCcGACAUCg -3' miRNA: 3'- -UCG--AGCUCCGGugaCUGGGaCUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 13432 | 0.66 | 0.628808 |
Target: 5'- cGC-CGGGGCCGCgcuaggcgUGACCgUcGGCGCg -3' miRNA: 3'- uCGaGCUCCGGUG--------ACUGGgA-CUGUGg -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 47641 | 0.66 | 0.618141 |
Target: 5'- gGGCagCGAGGCCcucuuugaacguGCUGuugaugGCCUUGGcCACCu -3' miRNA: 3'- -UCGa-GCUCCGG------------UGAC------UGGGACU-GUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 2128 | 0.66 | 0.618141 |
Target: 5'- cGC-CGAGGUCACgcaGACCC--GCACg -3' miRNA: 3'- uCGaGCUCCGGUGa--CUGGGacUGUGg -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 55503 | 0.66 | 0.618141 |
Target: 5'- cAGCgucaCGAGGCCcacagcgaACUGugCCcacaGCGCCg -3' miRNA: 3'- -UCGa---GCUCCGG--------UGACugGGac--UGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 21567 | 0.66 | 0.618141 |
Target: 5'- uGCgaacacugCGAGGUCgAUUGGCCaugcGACACCg -3' miRNA: 3'- uCGa-------GCUCCGG-UGACUGGga--CUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 57462 | 0.66 | 0.607485 |
Target: 5'- cGCUgCGgcAGGCCGgUGGCaCCgGACACg -3' miRNA: 3'- uCGA-GC--UCCGGUgACUG-GGaCUGUGg -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 48905 | 0.66 | 0.650141 |
Target: 5'- gAGCUcaaCGAG-CUGCUGGgCCUGACcgacGCCa -3' miRNA: 3'- -UCGA---GCUCcGGUGACUgGGACUG----UGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 17406 | 0.67 | 0.565143 |
Target: 5'- cAGCUacagagguucuaCGAGGCCGCacuGCCCgagGACAUUg -3' miRNA: 3'- -UCGA------------GCUCCGGUGac-UGGGa--CUGUGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 57969 | 0.67 | 0.565143 |
Target: 5'- uGgUCGAGGUCGCcggGAUCgUGACcCCg -3' miRNA: 3'- uCgAGCUCCGGUGa--CUGGgACUGuGG- -5' |
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23378 | 3' | -57.8 | NC_005259.1 | + | 3830 | 0.67 | 0.565143 |
Target: 5'- ---aCGGGGCCACgacgaUGuCCCUcGCACCg -3' miRNA: 3'- ucgaGCUCCGGUG-----ACuGGGAcUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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