Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23380 | 5' | -55.8 | NC_005259.1 | + | 61594 | 0.74 | 0.341334 |
Target: 5'- gCGGUgGGCCGGugG-GGUCGGCGg- -3' miRNA: 3'- aGCUAaCCGGCCugUgCCAGCUGCac -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 40462 | 0.7 | 0.514278 |
Target: 5'- gUGGUUGacguacuGCCGGGCcgcGCGGUUGACGUu -3' miRNA: 3'- aGCUAAC-------CGGCCUG---UGCCAGCUGCAc -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 58567 | 0.7 | 0.556581 |
Target: 5'- gCGGUUgGGCCGGAuCGCGGcgCGGCu-- -3' miRNA: 3'- aGCUAA-CCGGCCU-GUGCCa-GCUGcac -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 26810 | 0.7 | 0.556581 |
Target: 5'- cCGGUUGGCCGGuAC-CGG-CgGugGUGc -3' miRNA: 3'- aGCUAACCGGCC-UGuGCCaG-CugCAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 35658 | 0.7 | 0.56706 |
Target: 5'- gCGAgggUGGCCa-GCGCGGUgGugGUGc -3' miRNA: 3'- aGCUa--ACCGGccUGUGCCAgCugCAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 4990 | 0.68 | 0.652025 |
Target: 5'- cCGA-UGGCCGGGCACuacggcaagguGGUCGAg--- -3' miRNA: 3'- aGCUaACCGGCCUGUG-----------CCAGCUgcac -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 45619 | 0.68 | 0.673272 |
Target: 5'- cUCGAUgcccgccgGGCCGGGCAgcgcgcCGGUgcCGcCGUGa -3' miRNA: 3'- -AGCUAa-------CCGGCCUGU------GCCA--GCuGCAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 49038 | 0.67 | 0.744948 |
Target: 5'- gUCGAgucGGCaCGGACgaugacgugaucgGCGGUUGGCGc- -3' miRNA: 3'- -AGCUaa-CCG-GCCUG-------------UGCCAGCUGCac -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 26442 | 0.66 | 0.755991 |
Target: 5'- cCGAgcGGgCGGGC-CGGUgggcCGAUGUGg -3' miRNA: 3'- aGCUaaCCgGCCUGuGCCA----GCUGCAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 11590 | 0.66 | 0.785339 |
Target: 5'- gCGGgcUUGGCCGGAguuugcuggaACGcUCGAUGUGg -3' miRNA: 3'- aGCU--AACCGGCCUg---------UGCcAGCUGCAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 37462 | 0.66 | 0.794836 |
Target: 5'- cCGAUUGGCUGGcCACGccgccGUUGGC-UGa -3' miRNA: 3'- aGCUAACCGGCCuGUGC-----CAGCUGcAC- -5' |
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23380 | 5' | -55.8 | NC_005259.1 | + | 16785 | 0.66 | 0.794836 |
Target: 5'- cUCGug-GGUC-GACGCGGUCG-CGUa -3' miRNA: 3'- -AGCuaaCCGGcCUGUGCCAGCuGCAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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