Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23383 | 3' | -55.4 | NC_005259.1 | + | 13130 | 0.67 | 0.691113 |
Target: 5'- -aCCagCGcACgGUGUUCGGCUCGAUCAUg -3' miRNA: 3'- uaGGa-GC-UG-CGCAAGCUGGGCUAGUG- -5' |
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23383 | 3' | -55.4 | NC_005259.1 | + | 22633 | 0.66 | 0.7537 |
Target: 5'- cAUCUUCGcCGCGUcggUCGACgCCGccCACc -3' miRNA: 3'- -UAGGAGCuGCGCA---AGCUG-GGCuaGUG- -5' |
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23383 | 3' | -55.4 | NC_005259.1 | + | 42054 | 0.66 | 0.763777 |
Target: 5'- uGUCCUCGGgGUGggCGGCuaGGuaUCGCa -3' miRNA: 3'- -UAGGAGCUgCGCaaGCUGggCU--AGUG- -5' |
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23383 | 3' | -55.4 | NC_005259.1 | + | 46670 | 0.66 | 0.783528 |
Target: 5'- gGUUCUCGGCGaGcUCGGCCUGAgcCGCc -3' miRNA: 3'- -UAGGAGCUGCgCaAGCUGGGCUa-GUG- -5' |
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23383 | 3' | -55.4 | NC_005259.1 | + | 63994 | 0.66 | 0.7845 |
Target: 5'- uUCCUCG-CGCGUgacggcauaccaccgCG-CCCGAggCGCg -3' miRNA: 3'- uAGGAGCuGCGCAa--------------GCuGGGCUa-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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