Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2340 | 5' | -55.3 | NC_001416.1 | + | 13828 | 0.66 | 0.644383 |
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Target: 5'- aGCagUGCAGCGAa-CUGagCGCGGUGAg -3' miRNA: 3'- -CGg-ACGUCGUUgcGGCaaGUGCCACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 3906 | 0.66 | 0.644383 |
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Target: 5'- -gCUGCGGUAuaucGCuGCCGggCugGGUGu -3' miRNA: 3'- cgGACGUCGU----UG-CGGCaaGugCCACu -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 7826 | 0.67 | 0.598817 |
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Target: 5'- -gCUGCGGCGuggagacACGCUGacCAuCGGUGAg -3' miRNA: 3'- cgGACGUCGU-------UGCGGCaaGU-GCCACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 2088 | 0.67 | 0.577826 |
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Target: 5'- gGCCUcauccacacGCAGCAGCGuCUGUUCAuCGucGUGGc -3' miRNA: 3'- -CGGA---------CGUCGUUGC-GGCAAGU-GC--CACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 20756 | 0.68 | 0.56684 |
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Target: 5'- cGCUUGCuGCAACGCCaaagGCGGUu- -3' miRNA: 3'- -CGGACGuCGUUGCGGcaagUGCCAcu -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 4728 | 0.68 | 0.54504 |
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Target: 5'- uGCCggacaccgGCAGCAcggcgAUGCCGUUCAUGaccUGAu -3' miRNA: 3'- -CGGa-------CGUCGU-----UGCGGCAAGUGCc--ACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 7165 | 0.7 | 0.421815 |
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Target: 5'- aGCCaGCAGCAucaGCgGUgcugacugaaUCAUGGUGAa -3' miRNA: 3'- -CGGaCGUCGUug-CGgCA----------AGUGCCACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 5647 | 0.7 | 0.412302 |
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Target: 5'- gGCCagaaUGCGGCGG-GCCGUuuUCACGGUc- -3' miRNA: 3'- -CGG----ACGUCGUUgCGGCA--AGUGCCAcu -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 10834 | 0.71 | 0.402922 |
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Target: 5'- cGCCUGCAagcuGCGugGCCacgu-CGGUGAa -3' miRNA: 3'- -CGGACGU----CGUugCGGcaaguGCCACU- -5' |
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| 2340 | 5' | -55.3 | NC_001416.1 | + | 9659 | 1.11 | 0.000645 |
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Target: 5'- uGCCUGCAGCAACGCCGUUCACGGUGAu -3' miRNA: 3'- -CGGACGUCGUUGCGGCAAGUGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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