Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23403 | 3' | -51.2 | NC_005259.1 | + | 59302 | 0.66 | 0.94269 |
Target: 5'- aCGCugUCGugGCGGcaGCGGCGAGcucaGCCu -3' miRNA: 3'- -GUGuaAGUugCGUC--UGCCGUUC----UGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 11113 | 0.66 | 0.94269 |
Target: 5'- gUugGUgCGACGguGACGGUgcGAgCCGu -3' miRNA: 3'- -GugUAaGUUGCguCUGCCGuuCU-GGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 24222 | 0.66 | 0.93758 |
Target: 5'- -----aCAACGU-GAcCGGCGGGGCCGa -3' miRNA: 3'- guguaaGUUGCGuCU-GCCGUUCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 35362 | 0.66 | 0.932189 |
Target: 5'- --uGUUCAccaaGUAGGCGGUAGGuCCGa -3' miRNA: 3'- gugUAAGUug--CGUCUGCCGUUCuGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 43789 | 0.66 | 0.927094 |
Target: 5'- gGCAUgagCAGCGCGGGCaccgaguccuugccgGGCGuaccgccggacacaGGGCCGc -3' miRNA: 3'- gUGUAa--GUUGCGUCUG---------------CCGU--------------UCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 57452 | 0.66 | 0.926513 |
Target: 5'- gUugGUgCGACGCu-GCGGCA-GGCCGg -3' miRNA: 3'- -GugUAaGUUGCGucUGCCGUuCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 67155 | 0.66 | 0.926513 |
Target: 5'- cCGCuugUCGAUGguGGCGGCcauGCCGc -3' miRNA: 3'- -GUGua-AGUUGCguCUGCCGuucUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 24160 | 0.67 | 0.914311 |
Target: 5'- gCGCGaugUCuACGCcaaccacaccaaGGugGGCGAGAUCGa -3' miRNA: 3'- -GUGUa--AGuUGCG------------UCugCCGUUCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 65122 | 0.67 | 0.914311 |
Target: 5'- uCACcgUCGGCGCAccGGCGGCcacccCCGa -3' miRNA: 3'- -GUGuaAGUUGCGU--CUGCCGuucu-GGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 22083 | 0.67 | 0.914311 |
Target: 5'- aGCucaUCGAC-CAGAUGGCGGGcACCa -3' miRNA: 3'- gUGua-AGUUGcGUCUGCCGUUC-UGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 17594 | 0.67 | 0.914311 |
Target: 5'- gCGCAaccUCGGCGCgaugacgugGGGCGGCGAGGaCGg -3' miRNA: 3'- -GUGUa--AGUUGCG---------UCUGCCGUUCUgGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 7443 | 0.67 | 0.907786 |
Target: 5'- cCGCAUggCAGCGCcgaGGAUGGCuuGACUu -3' miRNA: 3'- -GUGUAa-GUUGCG---UCUGCCGuuCUGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 23614 | 0.67 | 0.907786 |
Target: 5'- cCGCGUggcggCAGC-CAGACGGCucGGugUGg -3' miRNA: 3'- -GUGUAa----GUUGcGUCUGCCGu-UCugGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 34558 | 0.67 | 0.907786 |
Target: 5'- uGCcgUCGGgGCcGACGGCGAGGuucUCGg -3' miRNA: 3'- gUGuaAGUUgCGuCUGCCGUUCU---GGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 52452 | 0.67 | 0.900982 |
Target: 5'- gAUGUUCG--GCGGAUGGC-AGACCa -3' miRNA: 3'- gUGUAAGUugCGUCUGCCGuUCUGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 24784 | 0.67 | 0.900982 |
Target: 5'- cCGCcgUauGCGCAGcAgGGcCAAGACCGa -3' miRNA: 3'- -GUGuaAguUGCGUC-UgCC-GUUCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 12028 | 0.67 | 0.891724 |
Target: 5'- -----aCggUGCGGGCGGCAaccgguacucaccgAGGCCGa -3' miRNA: 3'- guguaaGuuGCGUCUGCCGU--------------UCUGGC- -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 30475 | 0.68 | 0.870246 |
Target: 5'- aGCAgccUCGAgGUAGGCcagacccGGCGAGACCu -3' miRNA: 3'- gUGUa--AGUUgCGUCUG-------CCGUUCUGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 126 | 0.68 | 0.862913 |
Target: 5'- cCAUcaUCGAgGCcaACGGCAAGACCu -3' miRNA: 3'- -GUGuaAGUUgCGucUGCCGUUCUGGc -5' |
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23403 | 3' | -51.2 | NC_005259.1 | + | 3126 | 0.68 | 0.862913 |
Target: 5'- gCACcgUgGGCaGCGGACGGCAccgcccgugGGACUa -3' miRNA: 3'- -GUGuaAgUUG-CGUCUGCCGU---------UCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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