Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2341 | 5' | -48.2 | NC_001416.1 | + | 3082 | 0.7 | 0.828135 |
|
Target: 5'- ----cGUUAuuGCGuACCAGAUCGUCUGCg -3' miRNA: 3'- gaguuUAGU--UGC-UGGUCUAGCGGAUG- -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 7140 | 0.69 | 0.8893 |
|
Target: 5'- --uGAAUCAugGugaacucacgcGCCGGAUCGCCg-- -3' miRNA: 3'- gagUUUAGUugC-----------UGGUCUAGCGGaug -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 10617 | 1.1 | 0.003641 |
|
Target: 5'- aCUCAAAUCAACGACCAGAUCGCCUACc -3' miRNA: 3'- -GAGUUUAGUUGCUGGUCUAGCGGAUG- -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 17659 | 0.7 | 0.846934 |
|
Target: 5'- -aCGAuacCGACG-CCGGAUCGcCCUGCu -3' miRNA: 3'- gaGUUua-GUUGCuGGUCUAGC-GGAUG- -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 41615 | 0.67 | 0.956041 |
|
Target: 5'- --aAAAUCAACGcaaaaaAUCGGAcUUGCCUGCa -3' miRNA: 3'- gagUUUAGUUGC------UGGUCU-AGCGGAUG- -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 45276 | 0.68 | 0.929771 |
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Target: 5'- aUCGAcgCAACGAUguGcgccauuAUCGCCUGg -3' miRNA: 3'- gAGUUuaGUUGCUGguC-------UAGCGGAUg -5' |
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| 2341 | 5' | -48.2 | NC_001416.1 | + | 45621 | 0.68 | 0.904221 |
|
Target: 5'- aCUCAAAUCAACaggcGCCGGA-CGCUa-- -3' miRNA: 3'- -GAGUUUAGUUGc---UGGUCUaGCGGaug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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