Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23417 | 3' | -54.7 | NC_005259.1 | + | 65104 | 0.67 | 0.758485 |
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Target: 5'- cGUGgcacggUCAGUGcgucaCCGUCGGCGCaccggcgGCCa -3' miRNA: 3'- -CACaa----AGUCGCa----GGUAGCCGUGg------CGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 12156 | 0.67 | 0.768566 |
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Target: 5'- ----aUCAcCG-CCGaCGGCACCGCCc -3' miRNA: 3'- cacaaAGUcGCaGGUaGCCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 46572 | 0.66 | 0.778508 |
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Target: 5'- -gGUcgCGGCGUCgA-CcGCGCCGCCc -3' miRNA: 3'- caCAaaGUCGCAGgUaGcCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 42445 | 0.66 | 0.778508 |
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Target: 5'- cGUG-UUCGGCGUCCAacagucgccgacUaCGGCGagGCCc -3' miRNA: 3'- -CACaAAGUCGCAGGU------------A-GCCGUggCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 13447 | 0.66 | 0.778508 |
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Target: 5'- ------aGGCGUgaCCGUCGGCGCgGCg -3' miRNA: 3'- cacaaagUCGCA--GGUAGCCGUGgCGg -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 16681 | 0.66 | 0.788303 |
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Target: 5'- aUGUggUCGGCGaUCC-UgGGCaccgaggugACCGCCg -3' miRNA: 3'- cACAa-AGUCGC-AGGuAgCCG---------UGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 46913 | 0.66 | 0.788303 |
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Target: 5'- ------gAGCaGUCCG-CGuGCGCCGCCg -3' miRNA: 3'- cacaaagUCG-CAGGUaGC-CGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 23301 | 0.66 | 0.797939 |
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Target: 5'- -gGUaUCGGgGUCCggcgcgGUCGGUGUCGCCg -3' miRNA: 3'- caCAaAGUCgCAGG------UAGCCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 36236 | 0.66 | 0.807406 |
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Target: 5'- uUGgc-CAGCGcgagcgccUgCGUCGGCAgCGCCa -3' miRNA: 3'- cACaaaGUCGC--------AgGUAGCCGUgGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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