Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23419 | 3' | -53.3 | NC_005259.1 | + | 42778 | 0.76 | 0.322184 |
Target: 5'- --gGuCUCGGGGCCGCCAcCGAUCaCUGu -3' miRNA: 3'- guaC-GAGUUCCGGUGGUuGCUAG-GAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 17846 | 0.74 | 0.418445 |
Target: 5'- --gGC-CGAGGCCACCccguggucAGCGAUCCUu -3' miRNA: 3'- guaCGaGUUCCGGUGG--------UUGCUAGGAc -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 65413 | 0.73 | 0.428027 |
Target: 5'- uCAUGCUCAcGGUgGCCAaGCGAUggCCUGa -3' miRNA: 3'- -GUACGAGUuCCGgUGGU-UGCUA--GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 63550 | 0.73 | 0.468619 |
Target: 5'- gGUGCUCGGcGGCCACCGacgcgACGAgcgggauauacgccgCCUGg -3' miRNA: 3'- gUACGAGUU-CCGGUGGU-----UGCUa--------------GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 23348 | 0.72 | 0.477792 |
Target: 5'- --gGCUCGAGGCUGCCuGCGAggCCg- -3' miRNA: 3'- guaCGAGUUCCGGUGGuUGCUa-GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 57966 | 0.72 | 0.508984 |
Target: 5'- cCAUGgUCGAGGUCGCCGG-GAUCgUGa -3' miRNA: 3'- -GUACgAGUUCCGGUGGUUgCUAGgAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 50207 | 0.71 | 0.551826 |
Target: 5'- --cGCUCAAGcGCCGCCucgucaaucuCGAUCCg- -3' miRNA: 3'- guaCGAGUUC-CGGUGGuu--------GCUAGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 67608 | 0.71 | 0.562716 |
Target: 5'- aCGUGUUCGA-GCCACCGACGAaaaCCa- -3' miRNA: 3'- -GUACGAGUUcCGGUGGUUGCUa--GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 56092 | 0.7 | 0.595696 |
Target: 5'- gGUGCUCGucgGGGCCACC-GCGuggCCa- -3' miRNA: 3'- gUACGAGU---UCCGGUGGuUGCua-GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 61949 | 0.69 | 0.650051 |
Target: 5'- gGUGC-CGGGGCCACCGcagccucACGcgCCg- -3' miRNA: 3'- gUACGaGUUCCGGUGGU-------UGCuaGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 38038 | 0.69 | 0.706165 |
Target: 5'- gGUGUaCccGGCCACCAcaccCGAUCCUGc -3' miRNA: 3'- gUACGaGuuCCGGUGGUu---GCUAGGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 4471 | 0.68 | 0.748908 |
Target: 5'- --gGC-CAAGGCCAUCGGCGGUggaCCa- -3' miRNA: 3'- guaCGaGUUCCGGUGGUUGCUA---GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 40687 | 0.68 | 0.753089 |
Target: 5'- gGUGCUCAccggcccgaugaucgGGGugcCCGCCAccACGGUCCa- -3' miRNA: 3'- gUACGAGU---------------UCC---GGUGGU--UGCUAGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 60252 | 0.68 | 0.759322 |
Target: 5'- gCGUGCgggugCAAggcuGGCCACCAGCGcugCCg- -3' miRNA: 3'- -GUACGa----GUU----CCGGUGGUUGCua-GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 45315 | 0.67 | 0.768581 |
Target: 5'- --cGC-CGAGGCCGCCGccgaacuGCGuggCCUGc -3' miRNA: 3'- guaCGaGUUCCGGUGGU-------UGCua-GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 15094 | 0.67 | 0.769603 |
Target: 5'- uCAUGCgccgCAGGGCCAUCgaGugGAcCUUGg -3' miRNA: 3'- -GUACGa---GUUCCGGUGG--UugCUaGGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 36036 | 0.67 | 0.769603 |
Target: 5'- aCAUGaucagCGAGGCCACCcgcGCGAgcaccaCCUGc -3' miRNA: 3'- -GUACga---GUUCCGGUGGu--UGCUa-----GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 40035 | 0.67 | 0.799534 |
Target: 5'- gAUGCUuUGAGGUCGCCggUGAUCg-- -3' miRNA: 3'- gUACGA-GUUCCGGUGGuuGCUAGgac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 66840 | 0.67 | 0.809169 |
Target: 5'- --gGCUCAugccgcccgcacGGGCCACCGAuCGG-CCUu -3' miRNA: 3'- guaCGAGU------------UCCGGUGGUU-GCUaGGAc -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 16814 | 0.67 | 0.809169 |
Target: 5'- --cGCUCGGGGCCGgggUCA-UGAUCCg- -3' miRNA: 3'- guaCGAGUUCCGGU---GGUuGCUAGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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