Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23419 | 3' | -53.3 | NC_005259.1 | + | 45315 | 0.67 | 0.768581 |
Target: 5'- --cGC-CGAGGCCGCCGccgaacuGCGuggCCUGc -3' miRNA: 3'- guaCGaGUUCCGGUGGU-------UGCua-GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 55812 | 0.66 | 0.862659 |
Target: 5'- --gGCUCAcccucgggguAGGCCGCC-GCG-UCCUc -3' miRNA: 3'- guaCGAGU----------UCCGGUGGuUGCuAGGAc -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 24585 | 0.66 | 0.854307 |
Target: 5'- aCGUGCUCAAcGCUACCGgugACGGUUg-- -3' miRNA: 3'- -GUACGAGUUcCGGUGGU---UGCUAGgac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 28975 | 0.66 | 0.845717 |
Target: 5'- gGUGC-CGAGGCCGCgAGCGc-CCg- -3' miRNA: 3'- gUACGaGUUCCGGUGgUUGCuaGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 67676 | 0.66 | 0.845717 |
Target: 5'- uCAUGUUCuugaacGGGUCAUCAAUGAUCaUGa -3' miRNA: 3'- -GUACGAGu-----UCCGGUGGUUGCUAGgAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 8563 | 0.66 | 0.844845 |
Target: 5'- cCAUGCgccgcagCAccGCCACCGcccacgcACGAUCCg- -3' miRNA: 3'- -GUACGa------GUucCGGUGGU-------UGCUAGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 61148 | 0.66 | 0.836899 |
Target: 5'- cCGUGCcgacUCAAGGaCACCGACGGUguacgCCUc -3' miRNA: 3'- -GUACG----AGUUCCgGUGGUUGCUA-----GGAc -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 17742 | 0.66 | 0.827862 |
Target: 5'- -cUGCUCGacgcgauccgugAGGCCGCC-GCGcgCCg- -3' miRNA: 3'- guACGAGU------------UCCGGUGGuUGCuaGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 17821 | 0.66 | 0.818615 |
Target: 5'- --aGCUCAgcgAGGCCAUCGACGGg---- -3' miRNA: 3'- guaCGAGU---UCCGGUGGUUGCUaggac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 16814 | 0.67 | 0.809169 |
Target: 5'- --cGCUCGGGGCCGgggUCA-UGAUCCg- -3' miRNA: 3'- guaCGAGUUCCGGU---GGUuGCUAGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 66840 | 0.67 | 0.809169 |
Target: 5'- --gGCUCAugccgcccgcacGGGCCACCGAuCGG-CCUu -3' miRNA: 3'- guaCGAGU------------UCCGGUGGUU-GCUaGGAc -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 40035 | 0.67 | 0.799534 |
Target: 5'- gAUGCUuUGAGGUCGCCggUGAUCg-- -3' miRNA: 3'- gUACGA-GUUCCGGUGGuuGCUAGgac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 36036 | 0.67 | 0.769603 |
Target: 5'- aCAUGaucagCGAGGCCACCcgcGCGAgcaccaCCUGc -3' miRNA: 3'- -GUACga---GUUCCGGUGGu--UGCUa-----GGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 15094 | 0.67 | 0.769603 |
Target: 5'- uCAUGCgccgCAGGGCCAUCgaGugGAcCUUGg -3' miRNA: 3'- -GUACGa---GUUCCGGUGG--UugCUaGGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 42778 | 0.76 | 0.322184 |
Target: 5'- --gGuCUCGGGGCCGCCAcCGAUCaCUGu -3' miRNA: 3'- guaC-GAGUUCCGGUGGUuGCUAG-GAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 60252 | 0.68 | 0.759322 |
Target: 5'- gCGUGCgggugCAAggcuGGCCACCAGCGcugCCg- -3' miRNA: 3'- -GUACGa----GUU----CCGGUGGUUGCua-GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 40687 | 0.68 | 0.753089 |
Target: 5'- gGUGCUCAccggcccgaugaucgGGGugcCCGCCAccACGGUCCa- -3' miRNA: 3'- gUACGAGU---------------UCC---GGUGGU--UGCUAGGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 4471 | 0.68 | 0.748908 |
Target: 5'- --gGC-CAAGGCCAUCGGCGGUggaCCa- -3' miRNA: 3'- guaCGaGUUCCGGUGGUUGCUA---GGac -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 38038 | 0.69 | 0.706165 |
Target: 5'- gGUGUaCccGGCCACCAcaccCGAUCCUGc -3' miRNA: 3'- gUACGaGuuCCGGUGGUu---GCUAGGAC- -5' |
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23419 | 3' | -53.3 | NC_005259.1 | + | 61949 | 0.69 | 0.650051 |
Target: 5'- gGUGC-CGGGGCCACCGcagccucACGcgCCg- -3' miRNA: 3'- gUACGaGUUCCGGUGGU-------UGCuaGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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