Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2343 | 5' | -57.8 | NC_001416.1 | + | 10007 | 0.66 | 0.535106 |
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Target: 5'- gUGGU-GUACCGGCugucugguauguauGAGUuuguggugaauaaugCCCCUGAACa -3' miRNA: 3'- -ACUAgCGUGGCCG--------------UUCG---------------GGGGACUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 6073 | 0.66 | 0.5234 |
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Target: 5'- --cUCGuCGCUGGC-AGCCUCCggccaGAGCa -3' miRNA: 3'- acuAGC-GUGGCCGuUCGGGGGa----CUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 10601 | 0.66 | 0.512844 |
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Target: 5'- aGAUCGCcuACCGGUucAGCCaUCC-GGACu -3' miRNA: 3'- aCUAGCG--UGGCCGu-UCGG-GGGaCUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 20209 | 0.66 | 0.492004 |
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Target: 5'- cUGAUgccgcuucUGCGCCGGaggacGCUUCCUGAGCu -3' miRNA: 3'- -ACUA--------GCGUGGCCguu--CGGGGGACUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 1089 | 0.68 | 0.385459 |
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Target: 5'- -cGUCGCuGCUGGCGcuGGCCCCgUGGu- -3' miRNA: 3'- acUAGCG-UGGCCGU--UCGGGGgACUug -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 4321 | 0.69 | 0.341501 |
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Target: 5'- cGAUggaGCGCCGuGCAGccggucuuaaaccGCCCgCCUGGGCg -3' miRNA: 3'- aCUAg--CGUGGC-CGUU-------------CGGG-GGACUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 15151 | 0.71 | 0.253484 |
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Target: 5'- aUGAUCcgGCAauGGCGgcAGCCCCCaGGACa -3' miRNA: 3'- -ACUAG--CGUggCCGU--UCGGGGGaCUUG- -5' |
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| 2343 | 5' | -57.8 | NC_001416.1 | + | 10934 | 1.1 | 0.000352 |
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Target: 5'- gUGAUCGCACCGGCAAGCCCCCUGAACa -3' miRNA: 3'- -ACUAGCGUGGCCGUUCGGGGGACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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