Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23437 | 5' | -54.8 | NC_005259.1 | + | 4664 | 0.68 | 0.721688 |
Target: 5'- aCGGGcaCGGUGucuGCACUGGcUCGGGCc -3' miRNA: 3'- gGUUCcaGUCAC---UGUGGCCuAGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 7598 | 0.76 | 0.31416 |
Target: 5'- cUCAAGGUCGGUGACACCcucacccacgaGGGCc -3' miRNA: 3'- -GGUUCCAGUCACUGUGGccuag------CCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 9192 | 0.69 | 0.668973 |
Target: 5'- gUCGAGGUCGucauacGGgGCCGGAUCGaGCa -3' miRNA: 3'- -GGUUCCAGUca----CUgUGGCCUAGCcCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 9691 | 0.69 | 0.679622 |
Target: 5'- gUCGAGGUCGccgcucacugGGCACCGuccUCGGGCc -3' miRNA: 3'- -GGUUCCAGUca--------CUGUGGCcu-AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 10730 | 0.67 | 0.791644 |
Target: 5'- -uGAuGUCGGUGAUcaccgcgcgcuGCCGGGcgaucaucaugUCGGGCg -3' miRNA: 3'- ggUUcCAGUCACUG-----------UGGCCU-----------AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 12053 | 0.66 | 0.810367 |
Target: 5'- aCCGAGGccgagcucugCGGcaGACACCGaGAU-GGGCa -3' miRNA: 3'- -GGUUCCa---------GUCa-CUGUGGC-CUAgCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 17355 | 0.68 | 0.721688 |
Target: 5'- gUCGAGGUCGGcggcucGGCGCUGG-UCGuGGUc -3' miRNA: 3'- -GGUUCCAGUCa-----CUGUGGCCuAGC-CCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 17469 | 0.66 | 0.801089 |
Target: 5'- cCCAccGU-GGUGACGcCCGGccaugCGGGCa -3' miRNA: 3'- -GGUucCAgUCACUGU-GGCCua---GCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 18153 | 0.77 | 0.257554 |
Target: 5'- aCGAGaUCGGgcUGACGCCGGAUCuGGCa -3' miRNA: 3'- gGUUCcAGUC--ACUGUGGCCUAGcCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 23311 | 0.74 | 0.377966 |
Target: 5'- uCCGGcgcGGUCGGUGucgccgaGCCGGAuuacaUCGGGCu -3' miRNA: 3'- -GGUU---CCAGUCACug-----UGGCCU-----AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 24369 | 0.7 | 0.615427 |
Target: 5'- aCCGAGGUCuGGUGGCgcuACCGGAguacgUCGaacGCa -3' miRNA: 3'- -GGUUCCAG-UCACUG---UGGCCU-----AGCc--CG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 25204 | 0.68 | 0.700782 |
Target: 5'- aCCGGGGUCAGcGGCuacaagcCCGcGuuuuuccUCGGGCa -3' miRNA: 3'- -GGUUCCAGUCaCUGu------GGC-Cu------AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 25683 | 0.68 | 0.685992 |
Target: 5'- gUCGAGGcCGGUgucccaccccaaagGACACCGGccucgcuGUUGGGUa -3' miRNA: 3'- -GGUUCCaGUCA--------------CUGUGGCC-------UAGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 26817 | 0.67 | 0.762403 |
Target: 5'- gCCGGuaccGG-CGGUGGUGCCGGGgcggucugcUCGGGCa -3' miRNA: 3'- -GGUU----CCaGUCACUGUGGCCU---------AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 27478 | 0.7 | 0.609003 |
Target: 5'- gCCGGGGUCGGUGuggccaccgccccacGCGCCGaGGUCGa-- -3' miRNA: 3'- -GGUUCCAGUCAC---------------UGUGGC-CUAGCccg -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 31499 | 0.68 | 0.73202 |
Target: 5'- gCAGGGUCucaccGCGCCGGAcuugcuugaUCGGGg -3' miRNA: 3'- gGUUCCAGucac-UGUGGCCU---------AGCCCg -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 32895 | 0.66 | 0.819467 |
Target: 5'- aCCGGGGgcgagGGUGAgcCAcCCGGcccgcUCGGGCg -3' miRNA: 3'- -GGUUCCag---UCACU--GU-GGCCu----AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 34152 | 0.7 | 0.583392 |
Target: 5'- uCCGAGGUC-GUGGugccccaccagUGCCGGAacacacCGGGCa -3' miRNA: 3'- -GGUUCCAGuCACU-----------GUGGCCUa-----GCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 34868 | 0.67 | 0.752388 |
Target: 5'- uCCuc-GUCGGUGccGgACCGGGgugccUCGGGCa -3' miRNA: 3'- -GGuucCAGUCAC--UgUGGCCU-----AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 38627 | 0.71 | 0.551701 |
Target: 5'- aUCGAGGgCGGUGACGCCGaGUUGG-Cg -3' miRNA: 3'- -GGUUCCaGUCACUGUGGCcUAGCCcG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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