Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2345 | 5' | -58.5 | NC_001416.1 | + | 46238 | 0.69 | 0.341162 |
Target: 5'- aCCGCCuCCGgcguggauaauGCAGCCUCcccCCGAcUGGCa -3' miRNA: 3'- -GGCGGuGGU-----------UGUCGGGGc--GGUU-ACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 5835 | 0.69 | 0.349338 |
Target: 5'- gCGCCugCAAUGaCCCCGCUGAUG-Cu -3' miRNA: 3'- gGCGGugGUUGUcGGGGCGGUUACcG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15572 | 0.69 | 0.361016 |
Target: 5'- aUGCCAUCAGCgaagggccgauugaaGGUCCgGUgGAUGGCu -3' miRNA: 3'- gGCGGUGGUUG---------------UCGGGgCGgUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 9371 | 0.69 | 0.366103 |
Target: 5'- -aGUCACCAAUgugGGacgUCCGUCGAUGGCa -3' miRNA: 3'- ggCGGUGGUUG---UCg--GGGCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15659 | 0.68 | 0.383416 |
Target: 5'- gCCauCCACCGGaccuucaauCGGCCCuuCGCUGAUGGCa -3' miRNA: 3'- -GGc-GGUGGUU---------GUCGGG--GCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 7579 | 0.68 | 0.407634 |
Target: 5'- aCGCCGuucCCGGCAGCaCaaaUGCCAcagguucaucccgcGUGGCg -3' miRNA: 3'- gGCGGU---GGUUGUCG-Gg--GCGGU--------------UACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 17359 | 0.68 | 0.410383 |
Target: 5'- uUCGCCGUCAAagugcGCCCCGUuauccaCGAUGGCc -3' miRNA: 3'- -GGCGGUGGUUgu---CGGGGCG------GUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 45642 | 0.68 | 0.374692 |
Target: 5'- aCGCUACCAGCuucuuUCCCGUUggUGGg -3' miRNA: 3'- gGCGGUGGUUGuc---GGGGCGGuuACCg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 7630 | 0.68 | 0.392274 |
Target: 5'- aUGCUGCCAuu-GCCCgCGCCGAUGa- -3' miRNA: 3'- gGCGGUGGUuguCGGG-GCGGUUACcg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 17621 | 0.68 | 0.401263 |
Target: 5'- gCCG-CACCGGCAGCaCCGUCGA-GGa -3' miRNA: 3'- -GGCgGUGGUUGUCGgGGCGGUUaCCg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12862 | 0.67 | 0.429004 |
Target: 5'- aUGCCACCGGCGGUuaugucgguacaCCgGgCAgcAUGGCa -3' miRNA: 3'- gGCGGUGGUUGUCG------------GGgCgGU--UACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 24367 | 0.67 | 0.429004 |
Target: 5'- uCCGgCACaUAGCAGUCCUagggaCAGUGGCg -3' miRNA: 3'- -GGCgGUG-GUUGUCGGGGcg---GUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 14663 | 0.67 | 0.429004 |
Target: 5'- aCGgCACCugauACAGCCCCGUCGc---- -3' miRNA: 3'- gGCgGUGGu---UGUCGGGGCGGUuaccg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12343 | 0.67 | 0.448112 |
Target: 5'- uCUGCCuGCCGGucaguCAGCCCCcgGCUuuucgcaucAAUGGCg -3' miRNA: 3'- -GGCGG-UGGUU-----GUCGGGG--CGG---------UUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12220 | 0.67 | 0.448112 |
Target: 5'- uUGUCGCCAAguGCa--GCCAGcUGGCg -3' miRNA: 3'- gGCGGUGGUUguCGgggCGGUU-ACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 9288 | 0.67 | 0.448112 |
Target: 5'- aCGCCAgCAGCGccugcuGCCCCuGCUcuccgGGCa -3' miRNA: 3'- gGCGGUgGUUGU------CGGGG-CGGuua--CCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 19392 | 0.67 | 0.457842 |
Target: 5'- gCCGgaCCACCGACugugAGCCacuCCGgCC-AUGGCg -3' miRNA: 3'- -GGC--GGUGGUUG----UCGG---GGC-GGuUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 8346 | 0.67 | 0.471651 |
Target: 5'- gCCGCCACCuuucagggaugaacGCuGCCCCuuuuuacgacGCCugcGGCg -3' miRNA: 3'- -GGCGGUGGu-------------UGuCGGGG----------CGGuuaCCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 21209 | 0.66 | 0.497837 |
Target: 5'- uCgGCCuuuCCGGCAGgUgCGCCGaucccGUGGCc -3' miRNA: 3'- -GgCGGu--GGUUGUCgGgGCGGU-----UACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 10872 | 0.66 | 0.518415 |
Target: 5'- uUGCCGgugCGAUcaCCCUGCCGAUGGUg -3' miRNA: 3'- gGCGGUg--GUUGucGGGGCGGUUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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