Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2345 | 5' | -58.5 | NC_001416.1 | + | 11905 | 0.69 | 0.349337 |
Target: 5'- gCCGCCGCCAuCAGCguguuguaaUCCGCCu---GCa -3' miRNA: 3'- -GGCGGUGGUuGUCG---------GGGCGGuuacCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12220 | 0.67 | 0.448112 |
Target: 5'- uUGUCGCCAAguGCa--GCCAGcUGGCg -3' miRNA: 3'- gGCGGUGGUUguCGgggCGGUU-ACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12343 | 0.67 | 0.448112 |
Target: 5'- uCUGCCuGCCGGucaguCAGCCCCcgGCUuuucgcaucAAUGGCg -3' miRNA: 3'- -GGCGG-UGGUU-----GUCGGGG--CGG---------UUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12721 | 1.13 | 0.000201 |
Target: 5'- gCCGCCACCAACAGCCCCGCCAAUGGCg -3' miRNA: 3'- -GGCGGUGGUUGUCGGGGCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12862 | 0.67 | 0.429004 |
Target: 5'- aUGCCACCGGCGGUuaugucgguacaCCgGgCAgcAUGGCa -3' miRNA: 3'- gGCGGUGGUUGUCG------------GGgCgGU--UACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12943 | 0.73 | 0.185872 |
Target: 5'- gCUGCCcgguguACCGACAuaaCCGCCGGUGGCa -3' miRNA: 3'- -GGCGG------UGGUUGUcggGGCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 13525 | 0.66 | 0.508082 |
Target: 5'- aCGCUgGCCGACuGCUCCGCacg-GGUg -3' miRNA: 3'- gGCGG-UGGUUGuCGGGGCGguuaCCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 14310 | 0.66 | 0.528831 |
Target: 5'- gCGCUgguCCA-CAGCCaCC-CCGGUGGUc -3' miRNA: 3'- gGCGGu--GGUuGUCGG-GGcGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 14416 | 0.72 | 0.229211 |
Target: 5'- -aGCCGCCGGuCGGCCUCacucaGCCAG-GGCa -3' miRNA: 3'- ggCGGUGGUU-GUCGGGG-----CGGUUaCCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 14663 | 0.67 | 0.429004 |
Target: 5'- aCGgCACCugauACAGCCCCGUCGc---- -3' miRNA: 3'- gGCgGUGGu---UGUCGGGGCGGUuaccg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15110 | 0.72 | 0.206561 |
Target: 5'- aCGCCACCuGACuuGGCCCCGgCGAcucUGGg -3' miRNA: 3'- gGCGGUGG-UUG--UCGGGGCgGUU---ACCg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15117 | 0.7 | 0.315173 |
Target: 5'- -aGCCACCcuugcagcauggggGGCAGCCauugggGCCGGUGGUa -3' miRNA: 3'- ggCGGUGG--------------UUGUCGGgg----CGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15572 | 0.69 | 0.361016 |
Target: 5'- aUGCCAUCAGCgaagggccgauugaaGGUCCgGUgGAUGGCu -3' miRNA: 3'- gGCGGUGGUUG---------------UCGGGgCGgUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 15659 | 0.68 | 0.383416 |
Target: 5'- gCCauCCACCGGaccuucaauCGGCCCuuCGCUGAUGGCa -3' miRNA: 3'- -GGc-GGUGGUU---------GUCGGG--GCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 17359 | 0.68 | 0.410383 |
Target: 5'- uUCGCCGUCAAagugcGCCCCGUuauccaCGAUGGCc -3' miRNA: 3'- -GGCGGUGGUUgu---CGGGGCG------GUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 17621 | 0.68 | 0.401263 |
Target: 5'- gCCG-CACCGGCAGCaCCGUCGA-GGa -3' miRNA: 3'- -GGCgGUGGUUGUCGgGGCGGUUaCCg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 17957 | 0.71 | 0.256514 |
Target: 5'- cUCGCCcgACCGACGGCCUCcacgaaugccgauuuGCCAAcGGUg -3' miRNA: 3'- -GGCGG--UGGUUGUCGGGG---------------CGGUUaCCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 18050 | 0.69 | 0.357652 |
Target: 5'- uCCGUCAgcucgacuuuuuCCAGCAGCUCCuuGCCGAgaUGGg -3' miRNA: 3'- -GGCGGU------------GGUUGUCGGGG--CGGUU--ACCg -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 19392 | 0.67 | 0.457842 |
Target: 5'- gCCGgaCCACCGACugugAGCCacuCCGgCC-AUGGCg -3' miRNA: 3'- -GGC--GGUGGUUG----UCGG---GGC-GGuUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 20087 | 0.74 | 0.154044 |
Target: 5'- gCGCCGCCAGCAcgUCCGCCGGacaGGCu -3' miRNA: 3'- gGCGGUGGUUGUcgGGGCGGUUa--CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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