Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2345 | 5' | -58.5 | NC_001416.1 | + | 21209 | 0.66 | 0.497837 |
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Target: 5'- uCgGCCuuuCCGGCAGgUgCGCCGaucccGUGGCc -3' miRNA: 3'- -GgCGGu--GGUUGUCgGgGCGGU-----UACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 24367 | 0.67 | 0.429004 |
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Target: 5'- uCCGgCACaUAGCAGUCCUagggaCAGUGGCg -3' miRNA: 3'- -GGCgGUG-GUUGUCGGGGcg---GUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 33394 | 0.71 | 0.260437 |
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Target: 5'- -aGCCAgCAACAGgccaCCCUGC-AAUGGCa -3' miRNA: 3'- ggCGGUgGUUGUC----GGGGCGgUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 39923 | 0.69 | 0.357652 |
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Target: 5'- cCCGgCACCAugcaacaaACuGCCCgGgUGAUGGCa -3' miRNA: 3'- -GGCgGUGGU--------UGuCGGGgCgGUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 45642 | 0.68 | 0.374692 |
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Target: 5'- aCGCUACCAGCuucuuUCCCGUUggUGGg -3' miRNA: 3'- gGCGGUGGUUGuc---GGGGCGGuuACCg -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 46238 | 0.69 | 0.341162 |
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Target: 5'- aCCGCCuCCGgcguggauaauGCAGCCUCcccCCGAcUGGCa -3' miRNA: 3'- -GGCGGuGGU-----------UGUCGGGGc--GGUU-ACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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