Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2345 | 5' | -58.5 | NC_001416.1 | + | 4876 | 0.73 | 0.180993 |
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Target: 5'- cCCGCCACCA----UCCCGCCg--GGCg -3' miRNA: 3'- -GGCGGUGGUugucGGGGCGGuuaCCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 4835 | 0.66 | 0.487686 |
|
Target: 5'- gCCGuCCACCAucggaucgcugGCAGCCUguUGCagAcgGGCg -3' miRNA: 3'- -GGC-GGUGGU-----------UGUCGGG--GCGg-UuaCCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 4748 | 0.66 | 0.487686 |
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Target: 5'- cCCGUCugCAACAggcuGCCagCGauCCGAUGGUg -3' miRNA: 3'- -GGCGGugGUUGU----CGGg-GC--GGUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 2256 | 0.66 | 0.498857 |
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Target: 5'- -gGCCACCGGCuuuccguagacggauGCCCCuuUAAUGGg -3' miRNA: 3'- ggCGGUGGUUGu--------------CGGGGcgGUUACCg -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 1090 | 0.73 | 0.180993 |
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Target: 5'- gUCGCUGCUGGCgcuGGCCCCGUgGuAUGGCa -3' miRNA: 3'- -GGCGGUGGUUG---UCGGGGCGgU-UACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 652 | 0.7 | 0.295043 |
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Target: 5'- gCCGCugCACCGACAGggggagCCCGUCGAgaauacUGGCa -3' miRNA: 3'- -GGCG--GUGGUUGUCg-----GGGCGGUU------ACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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