Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2345 | 5' | -58.5 | NC_001416.1 | + | 18050 | 0.69 | 0.357652 |
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Target: 5'- uCCGUCAgcucgacuuuuuCCAGCAGCUCCuuGCCGAgaUGGg -3' miRNA: 3'- -GGCGGU------------GGUUGUCGGGG--CGGUU--ACCg -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 11905 | 0.69 | 0.349337 |
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Target: 5'- gCCGCCGCCAuCAGCguguuguaaUCCGCCu---GCa -3' miRNA: 3'- -GGCGGUGGUuGUCG---------GGGCGGuuacCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 9933 | 0.7 | 0.317473 |
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Target: 5'- aUCGCCACCAGaaacGCgCCGguuCUGAUGGCg -3' miRNA: 3'- -GGCGGUGGUUgu--CGgGGC---GGUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 10985 | 0.7 | 0.30238 |
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Target: 5'- gCCaGCgCACCggUcGCCaCCGCCAgcgagGUGGCc -3' miRNA: 3'- -GG-CG-GUGGuuGuCGG-GGCGGU-----UACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 652 | 0.7 | 0.295043 |
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Target: 5'- gCCGCugCACCGACAGggggagCCCGUCGAgaauacUGGCa -3' miRNA: 3'- -GGCG--GUGGUUGUCg-----GGGCGGUU------ACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 17957 | 0.71 | 0.256514 |
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Target: 5'- cUCGCCcgACCGACGGCCUCcacgaaugccgauuuGCCAAcGGUg -3' miRNA: 3'- -GGCGG--UGGUUGUCGGGG---------------CGGUUaCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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