Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2345 | 5' | -58.5 | NC_001416.1 | + | 5057 | 0.66 | 0.487686 |
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Target: 5'- aCCGUaauUACUGugAGCCaucaugaCGCCGAUGGa -3' miRNA: 3'- -GGCG---GUGGUugUCGGg------GCGGUUACCg -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 21209 | 0.66 | 0.497837 |
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Target: 5'- uCgGCCuuuCCGGCAGgUgCGCCGaucccGUGGCc -3' miRNA: 3'- -GgCGGu--GGUUGUCgGgGCGGU-----UACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 2256 | 0.66 | 0.498857 |
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Target: 5'- -gGCCACCGGCuuuccguagacggauGCCCCuuUAAUGGg -3' miRNA: 3'- ggCGGUGGUUGu--------------CGGGGcgGUUACCg -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 13525 | 0.66 | 0.508082 |
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Target: 5'- aCGCUgGCCGACuGCUCCGCacg-GGUg -3' miRNA: 3'- gGCGG-UGGUUGuCGGGGCGguuaCCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 10872 | 0.66 | 0.518415 |
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Target: 5'- uUGCCGgugCGAUcaCCCUGCCGAUGGUg -3' miRNA: 3'- gGCGGUg--GUUGucGGGGCGGUUACCG- -5' |
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| 2345 | 5' | -58.5 | NC_001416.1 | + | 14310 | 0.66 | 0.528831 |
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Target: 5'- gCGCUgguCCA-CAGCCaCC-CCGGUGGUc -3' miRNA: 3'- gGCGGu--GGUuGUCGG-GGcGGUUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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