Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2345 | 5' | -58.5 | NC_001416.1 | + | 4876 | 0.73 | 0.180993 |
Target: 5'- cCCGCCACCA----UCCCGCCg--GGCg -3' miRNA: 3'- -GGCGGUGGUugucGGGGCGGuuaCCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 1090 | 0.73 | 0.180993 |
Target: 5'- gUCGCUGCUGGCgcuGGCCCCGUgGuAUGGCa -3' miRNA: 3'- -GGCGGUGGUUG---UCGGGGCGgU-UACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 20087 | 0.74 | 0.154044 |
Target: 5'- gCGCCGCCAGCAcgUCCGCCGGacaGGCu -3' miRNA: 3'- gGCGGUGGUUGUcgGGGCGGUUa--CCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 5896 | 0.75 | 0.134422 |
Target: 5'- gCaCCACCGACGGUgCUGCCGuUGGCa -3' miRNA: 3'- gGcGGUGGUUGUCGgGGCGGUuACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 5979 | 0.77 | 0.101919 |
Target: 5'- aCCGCaagaauGCCAACGGCagcaCCGUCGGUGGUg -3' miRNA: 3'- -GGCGg-----UGGUUGUCGg---GGCGGUUACCG- -5' |
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2345 | 5' | -58.5 | NC_001416.1 | + | 12721 | 1.13 | 0.000201 |
Target: 5'- gCCGCCACCAACAGCCCCGCCAAUGGCg -3' miRNA: 3'- -GGCGGUGGUUGUCGGGGCGGUUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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