Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23458 | 3' | -56.7 | NC_005259.1 | + | 66901 | 0.68 | 0.578557 |
Target: 5'- gUGAacGGCGGuagaucCGG-CAGcGCGCGGUGCu -3' miRNA: 3'- gACUa-CCGCU------GCUaGUC-CGCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 58762 | 0.69 | 0.536601 |
Target: 5'- ----cGGCGaACGAcgcugCGGGCGCuGUGCg -3' miRNA: 3'- gacuaCCGC-UGCUa----GUCCGCGcCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 26377 | 0.69 | 0.516014 |
Target: 5'- -cGGUGGCGagccuguugcucGCGAUuccgacgcCGGGCGCGGcggUGCc -3' miRNA: 3'- gaCUACCGC------------UGCUA--------GUCCGCGCC---ACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 58319 | 0.7 | 0.495752 |
Target: 5'- uUGAUGGUGAUcucgggcagcgaGAUCAGGU-CGGUGg -3' miRNA: 3'- gACUACCGCUG------------CUAGUCCGcGCCACg -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 28339 | 0.7 | 0.475858 |
Target: 5'- ---uUGGCGGCGAaaUCgAGGCcaCGGUGCu -3' miRNA: 3'- gacuACCGCUGCU--AG-UCCGc-GCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 68682 | 0.7 | 0.469967 |
Target: 5'- -gGAUGGCcuucucgaugaucgaGGCGggCAGcGCGuCGGUGCc -3' miRNA: 3'- gaCUACCG---------------CUGCuaGUC-CGC-GCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 60566 | 0.71 | 0.399742 |
Target: 5'- -cGGUGcCGACGGUCAGGCgGCccacggcgagucgGGUGCc -3' miRNA: 3'- gaCUACcGCUGCUAGUCCG-CG-------------CCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 41634 | 0.71 | 0.391774 |
Target: 5'- aUGGUGGCGGugcCGGUCugcGGGCGCucGGUGa -3' miRNA: 3'- gACUACCGCU---GCUAG---UCCGCG--CCACg -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 53729 | 0.72 | 0.365971 |
Target: 5'- gCUGAUGGUGAUGucgcCGGGUGUccacucGGUGCc -3' miRNA: 3'- -GACUACCGCUGCua--GUCCGCG------CCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 14651 | 0.73 | 0.333433 |
Target: 5'- -cGAUGGCGugGGcucagcccgacUCGGGC-CGcGUGCa -3' miRNA: 3'- gaCUACCGCugCU-----------AGUCCGcGC-CACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 66191 | 0.74 | 0.288699 |
Target: 5'- gCUGccGGUGcaguACgGGUCAGGCGCGGUcGCg -3' miRNA: 3'- -GACuaCCGC----UG-CUAGUCCGCGCCA-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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