miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23460 3' -60.3 NC_005259.1 + 63686 0.74 0.175658
Target:  5'- cGGCGCGCCGGGgaucugcgcCUGCCCaGCggggaUCGGGg -3'
miRNA:   3'- -CCGCGCGGCCU---------GAUGGGcUG-----AGUCCa -5'
23460 3' -60.3 NC_005259.1 + 65892 0.76 0.12514
Target:  5'- cGGCGggacgguccCGCCGGACaagagauaaguUACCCGACUUGGGa -3'
miRNA:   3'- -CCGC---------GCGGCCUG-----------AUGGGCUGAGUCCa -5'
23460 3' -60.3 NC_005259.1 + 65984 0.68 0.414484
Target:  5'- aGGCGCGCCGaGcaGCcGCUCGACgu-GGUg -3'
miRNA:   3'- -CCGCGCGGC-C--UGaUGGGCUGaguCCA- -5'
23460 3' -60.3 NC_005259.1 + 67425 0.66 0.508565
Target:  5'- cGGgGUGCCGGGCUcacGCuuGAgcgcgUCGGGg -3'
miRNA:   3'- -CCgCGCGGCCUGA---UGggCUg----AGUCCa -5'
23460 3' -60.3 NC_005259.1 + 68711 0.68 0.40567
Target:  5'- aGCGCGUCGG--UGCCCGAgaaCUgGGGUc -3'
miRNA:   3'- cCGCGCGGCCugAUGGGCU---GAgUCCA- -5'
23460 3' -60.3 NC_005259.1 + 68873 0.66 0.498722
Target:  5'- -cCGCGCCGGGC-GCgCCGAUg-AGGUa -3'
miRNA:   3'- ccGCGCGGCCUGaUG-GGCUGagUCCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.