Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
23460 | 3' | -60.3 | NC_005259.1 | + | 15793 | 0.67 | 0.479298 |
Target: 5'- uGGC-CGCCGGGaucgaccGCCCGACaUCcaAGGUg -3' miRNA: 3'- -CCGcGCGGCCUga-----UGGGCUG-AG--UCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 68873 | 0.66 | 0.498722 |
Target: 5'- -cCGCGCCGGGC-GCgCCGAUg-AGGUa -3' miRNA: 3'- ccGCGCGGCCUGaUG-GGCUGagUCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 38027 | 0.66 | 0.508565 |
Target: 5'- aGGCccGCGuuGGugUACCCGGC-CAc-- -3' miRNA: 3'- -CCG--CGCggCCugAUGGGCUGaGUcca -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 67425 | 0.66 | 0.508565 |
Target: 5'- cGGgGUGCCGGGCUcacGCuuGAgcgcgUCGGGg -3' miRNA: 3'- -CCgCGCGGCCUGA---UGggCUg----AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 61506 | 0.66 | 0.548693 |
Target: 5'- aGGCGC-UCGGugUGCCgguCGACcucgUCGGGa -3' miRNA: 3'- -CCGCGcGGCCugAUGG---GCUG----AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 7448 | 0.66 | 0.558887 |
Target: 5'- uGGCaGCGCCGaggauggcuuGACUugacaauCCCGACauaggCGGGUa -3' miRNA: 3'- -CCG-CGCGGC----------CUGAu------GGGCUGa----GUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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