Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23460 | 3' | -60.3 | NC_005259.1 | + | 30796 | 0.71 | 0.288433 |
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Target: 5'- cGCGUaccGCCGGAacACCUGAgUCGGGUa -3' miRNA: 3'- cCGCG---CGGCCUgaUGGGCUgAGUCCA- -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 14654 | 0.71 | 0.26203 |
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Target: 5'- uGGCGU---GGGCUcaGCCCGACUCGGGc -3' miRNA: 3'- -CCGCGcggCCUGA--UGGGCUGAGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 63686 | 0.74 | 0.175658 |
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Target: 5'- cGGCGCGCCGGGgaucugcgcCUGCCCaGCggggaUCGGGg -3' miRNA: 3'- -CCGCGCGGCCU---------GAUGGGcUG-----AGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 57694 | 0.75 | 0.15437 |
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Target: 5'- uGGCcagguUGCCGGGCuUGCCCGACUCgcGGGUc -3' miRNA: 3'- -CCGc----GCGGCCUG-AUGGGCUGAG--UCCA- -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 65892 | 0.76 | 0.12514 |
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Target: 5'- cGGCGggacgguccCGCCGGACaagagauaaguUACCCGACUUGGGa -3' miRNA: 3'- -CCGC---------GCGGCCUG-----------AUGGGCUGAGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 50410 | 0.76 | 0.118678 |
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Target: 5'- aGCGCGCCGGugcCUugaCCGGCUCGGGc -3' miRNA: 3'- cCGCGCGGCCu--GAug-GGCUGAGUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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