Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23463 | 3' | -53.5 | NC_005259.1 | + | 58433 | 0.7 | 0.65064 |
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Target: 5'- gGCAgauugCGCUGAUCg-GUGGCCuUGGUg -3' miRNA: 3'- -CGUaa---GCGGCUAGagCACCGGcAUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 60079 | 0.67 | 0.824307 |
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Target: 5'- gGCGgcUCGCCGAcuuUCUCGaUGGCg--AGCu -3' miRNA: 3'- -CGUa-AGCGGCU---AGAGC-ACCGgcaUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 60213 | 0.73 | 0.480597 |
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Target: 5'- ---cUCGCCGAUCUCGUcGGCgcugAGCu -3' miRNA: 3'- cguaAGCGGCUAGAGCA-CCGgca-UCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 60469 | 0.67 | 0.833246 |
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Target: 5'- ----aCGCUGGUCUCGUaGGCaCGcaccAGCg -3' miRNA: 3'- cguaaGCGGCUAGAGCA-CCG-GCa---UCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 60500 | 0.66 | 0.849653 |
|
Target: 5'- aGCAcgCGCCGGUgUUGUcguagugcgccauGGCCG-GGCc -3' miRNA: 3'- -CGUaaGCGGCUAgAGCA-------------CCGGCaUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 61263 | 0.67 | 0.841114 |
|
Target: 5'- aGCAUgcgaccgUCGCUGGUCUCGauGCCGacgaacgcgAGCg -3' miRNA: 3'- -CGUA-------AGCGGCUAGAGCacCGGCa--------UCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 67054 | 0.7 | 0.639764 |
|
Target: 5'- aGCAcccgugCGCCGAUCUCaagGGCC--AGCg -3' miRNA: 3'- -CGUaa----GCGGCUAGAGca-CCGGcaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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