Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23463 | 3' | -53.5 | NC_005259.1 | + | 45514 | 0.69 | 0.725731 |
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Target: 5'- gGCcaccgCGCCGGUgUUGgccGCCGUGGCu -3' miRNA: 3'- -CGuaa--GCGGCUAgAGCac-CGGCAUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 37582 | 0.69 | 0.73514 |
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Target: 5'- cGCccgCGCCGAUCugcaagcugaacgUCG-GGCCG-AGCu -3' miRNA: 3'- -CGuaaGCGGCUAG-------------AGCaCCGGCaUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 4972 | 0.68 | 0.786672 |
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Target: 5'- gGUAUcCGCCGAgUUCGgccgaUGGCCG-GGCa -3' miRNA: 3'- -CGUAaGCGGCUaGAGC-----ACCGGCaUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 49555 | 0.67 | 0.81517 |
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Target: 5'- ----gUGCCGGgcugUUCGUcGGUCGUGGCc -3' miRNA: 3'- cguaaGCGGCUa---GAGCA-CCGGCAUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 13400 | 0.67 | 0.81517 |
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Target: 5'- cGCAUUCGCuCGGccUUCcUGGCCGgGGUc -3' miRNA: 3'- -CGUAAGCG-GCUa-GAGcACCGGCaUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 15421 | 0.67 | 0.824307 |
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Target: 5'- uGCGUgccaccaagGCCGAUCUCGaguaucUGGCCGagaucaacccguUGGCc -3' miRNA: 3'- -CGUAag-------CGGCUAGAGC------ACCGGC------------AUCG- -5' |
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| 23463 | 3' | -53.5 | NC_005259.1 | + | 31859 | 0.66 | 0.874671 |
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Target: 5'- cGCGUcaUUGgCGAUCUUGUcGGUCGUGa- -3' miRNA: 3'- -CGUA--AGCgGCUAGAGCA-CCGGCAUcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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