miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23482 5' -53.1 NC_005259.1 + 61287 0.66 0.884929
Target:  5'- -aUGCcGACGAACGCGagCGGCugauCGGUg -3'
miRNA:   3'- caGCGcUUGCUUGCGUa-GCUGu---GCCA- -5'
23482 5' -53.1 NC_005259.1 + 66898 0.66 0.882703
Target:  5'- aUCGUGAACGGcgguagauccggcaGCGCGcggugcUCGAC-CGGg -3'
miRNA:   3'- cAGCGCUUGCU--------------UGCGU------AGCUGuGCCa -5'
23482 5' -53.1 NC_005259.1 + 55012 0.66 0.877421
Target:  5'- cUCGCuuGGCGGuCGUcUCGGCACGGg -3'
miRNA:   3'- cAGCGc-UUGCUuGCGuAGCUGUGCCa -5'
23482 5' -53.1 NC_005259.1 + 40366 0.66 0.877421
Target:  5'- -cCGCGc-CGAGgGCAugaggucuuccaUCGACACGGg -3'
miRNA:   3'- caGCGCuuGCUUgCGU------------AGCUGUGCCa -5'
23482 5' -53.1 NC_005259.1 + 6382 0.66 0.877421
Target:  5'- -gCGCGAugGucAACGUcauuuUCGAgGCGGUg -3'
miRNA:   3'- caGCGCUugC--UUGCGu----AGCUgUGCCA- -5'
23482 5' -53.1 NC_005259.1 + 57064 0.66 0.899178
Target:  5'- -cCGUGAucggguCGAACGCGUCGAgGauguCGGg -3'
miRNA:   3'- caGCGCUu-----GCUUGCGUAGCUgU----GCCa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.