Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23482 | 5' | -53.1 | NC_005259.1 | + | 61287 | 0.66 | 0.884929 |
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Target: 5'- -aUGCcGACGAACGCGagCGGCugauCGGUg -3' miRNA: 3'- caGCGcUUGCUUGCGUa-GCUGu---GCCA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 63470 | 0.7 | 0.686379 |
|
Target: 5'- -cCGCG--UGAuCGCGUCGAUACGGg -3' miRNA: 3'- caGCGCuuGCUuGCGUAGCUGUGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 66898 | 0.66 | 0.882703 |
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Target: 5'- aUCGUGAACGGcgguagauccggcaGCGCGcggugcUCGAC-CGGg -3' miRNA: 3'- cAGCGCUUGCU--------------UGCGU------AGCUGuGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 66988 | 0.66 | 0.892183 |
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Target: 5'- aUCGCGuuguACGccucACGCA-CGACACGcGUg -3' miRNA: 3'- cAGCGCu---UGCu---UGCGUaGCUGUGC-CA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 68300 | 0.7 | 0.675566 |
|
Target: 5'- aUCGgGAuCGGGCGCGUCcGGCgACGGg -3' miRNA: 3'- cAGCgCUuGCUUGCGUAG-CUG-UGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 68523 | 0.71 | 0.63201 |
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Target: 5'- -cUGcCGAGCGAgcugcccgccACGCGUCGACAuCGGg -3' miRNA: 3'- caGC-GCUUGCU----------UGCGUAGCUGU-GCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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