Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23482 | 5' | -53.1 | NC_005259.1 | + | 40366 | 0.66 | 0.877421 |
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Target: 5'- -cCGCGc-CGAGgGCAugaggucuuccaUCGACACGGg -3' miRNA: 3'- caGCGCuuGCUUgCGU------------AGCUGUGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 40064 | 0.66 | 0.89849 |
|
Target: 5'- gGUCGCG-AUGAcccaugggccgacGCGCAcCGAgCGCGGa -3' miRNA: 3'- -CAGCGCuUGCU-------------UGCGUaGCU-GUGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 36243 | 0.68 | 0.780082 |
|
Target: 5'- -gCGCGAGCGccUGCGUCGGCAgcgccacgcCGGa -3' miRNA: 3'- caGCGCUUGCuuGCGUAGCUGU---------GCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 24031 | 0.67 | 0.81829 |
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Target: 5'- --gGCGAAauggaaccgcCGcAACGuCGUCGGCGCGGUg -3' miRNA: 3'- cagCGCUU----------GC-UUGC-GUAGCUGUGCCA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 6382 | 0.66 | 0.877421 |
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Target: 5'- -gCGCGAugGucAACGUcauuuUCGAgGCGGUg -3' miRNA: 3'- caGCGCUugC--UUGCGu----AGCUgUGCCA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 4345 | 0.69 | 0.718471 |
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Target: 5'- cGUCGCGGugAUGAGCGCcggaCGGCACGc- -3' miRNA: 3'- -CAGCGCU--UGCUUGCGua--GCUGUGCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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