Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23482 | 5' | -53.1 | NC_005259.1 | + | 68300 | 0.7 | 0.675566 |
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Target: 5'- aUCGgGAuCGGGCGCGUCcGGCgACGGg -3' miRNA: 3'- cAGCgCUuGCUUGCGUAG-CUG-UGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 68523 | 0.71 | 0.63201 |
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Target: 5'- -cUGcCGAGCGAgcugcccgccACGCGUCGACAuCGGg -3' miRNA: 3'- caGC-GCUUGCU----------UGCGUAGCUGU-GCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 58760 | 0.71 | 0.614552 |
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Target: 5'- uUCgGCGAACGAcgcugcgggcgcuguGCGCcgGUCGAgGCGGUg -3' miRNA: 3'- cAG-CGCUUGCU---------------UGCG--UAGCUgUGCCA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 57007 | 0.72 | 0.556125 |
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Target: 5'- -gUGCGAGCaccucAGCGCGgcUCGGCGCGGUg -3' miRNA: 3'- caGCGCUUGc----UUGCGU--AGCUGUGCCA- -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 54685 | 0.73 | 0.493261 |
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Target: 5'- --gGCGAGCGAGacCGUGUCGAgGCGGg -3' miRNA: 3'- cagCGCUUGCUU--GCGUAGCUgUGCCa -5' |
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| 23482 | 5' | -53.1 | NC_005259.1 | + | 51659 | 0.76 | 0.361542 |
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Target: 5'- gGUUGcCGcGCGGGCGCGUCGgGCACGGg -3' miRNA: 3'- -CAGC-GCuUGCUUGCGUAGC-UGUGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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