Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23489 | 5' | -56.4 | NC_005259.1 | + | 34233 | 0.73 | 0.296217 |
Target: 5'- cUGCCgGucuuGCCGUugaacguguccgacACAUCGGGCUCAGc -3' miRNA: 3'- -ACGGgCu---CGGCA--------------UGUAGCUCGAGUCu -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 1413 | 0.75 | 0.250662 |
Target: 5'- cGaCCCGAGCaCGgcaUACGUCGuGCUCGGGc -3' miRNA: 3'- aC-GGGCUCG-GC---AUGUAGCuCGAGUCU- -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 10350 | 0.75 | 0.23823 |
Target: 5'- gGCagCGGGCuCG-ACAUCGGGCUCGGAa -3' miRNA: 3'- aCGg-GCUCG-GCaUGUAGCUCGAGUCU- -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 23324 | 0.77 | 0.188551 |
Target: 5'- gUGUcgCCGAGCCGgauUACAUCGGGCUCgAGGc -3' miRNA: 3'- -ACG--GGCUCGGC---AUGUAGCUCGAG-UCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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