Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23490 | 5' | -54.9 | NC_005259.1 | + | 22226 | 0.66 | 0.799527 |
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Target: 5'- cGCcGCGAGGcuGUCGGUuuccGUCGaCGCa -3' miRNA: 3'- cCGcCGCUCCuuUAGCUA----CAGC-GCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 21008 | 0.66 | 0.799527 |
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Target: 5'- gGGCGGCGAcccugaccGGcagcuGAcCGAUGaccggCGCGCGu -3' miRNA: 3'- -CCGCCGCU--------CCu----UUaGCUACa----GCGCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 19217 | 0.67 | 0.750672 |
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Target: 5'- gGGCGGCGAcguGGGAcacCGA-GUCG-GCGa -3' miRNA: 3'- -CCGCCGCU---CCUUua-GCUaCAGCgCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 17618 | 0.71 | 0.560287 |
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Target: 5'- gGGCGGCGAGGA---CGGUuUCGCa-- -3' miRNA: 3'- -CCGCCGCUCCUuuaGCUAcAGCGcgc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 17182 | 0.68 | 0.688383 |
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Target: 5'- aGGCGGCGcAGGAAGccgCGcGUGagGCGgGu -3' miRNA: 3'- -CCGCCGC-UCCUUUa--GC-UACagCGCgC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 11779 | 0.66 | 0.835683 |
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Target: 5'- -aCGGCGAGGGAGUCccacacaUCGgGCGc -3' miRNA: 3'- ccGCCGCUCCUUUAGcuac---AGCgCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 11720 | 0.76 | 0.282973 |
|
Target: 5'- gGGCcacGGCGAGGcucAGAUCGAUGgUGCGCa -3' miRNA: 3'- -CCG---CCGCUCC---UUUAGCUACaGCGCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 9498 | 0.72 | 0.488235 |
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Target: 5'- --aGGCGAGGAAcgUGcgcucGUCGCGCGc -3' miRNA: 3'- ccgCCGCUCCUUuaGCua---CAGCGCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 6655 | 0.67 | 0.750672 |
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Target: 5'- -aCGGuCGAGGguGUCcGUGaCGCGCGa -3' miRNA: 3'- ccGCC-GCUCCuuUAGcUACaGCGCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 3174 | 0.67 | 0.760714 |
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Target: 5'- -cCGGCGAugucaaGGucGUCGGUG-CGCGCa -3' miRNA: 3'- ccGCCGCU------CCuuUAGCUACaGCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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