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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23502 | 3' | -50.8 | NC_005259.1 | + | 57877 | 0.73 | 0.634132 |
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Target: 5'- cCUCGAUGugGcggugcGCGACAgaccccGCUUGAGAc -3' miRNA: 3'- -GAGCUACugC------UGCUGUaa----CGGACUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 18347 | 0.72 | 0.722197 |
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Target: 5'- gUCGAcgccGACGGCGACAUguggcacaUGCCcGAGGg -3' miRNA: 3'- gAGCUa---CUGCUGCUGUA--------ACGGaCUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 5565 | 0.69 | 0.841423 |
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Target: 5'- -gUGGUGACGGCGAgaCcgUGCCgugcUGGGAa -3' miRNA: 3'- gaGCUACUGCUGCU--GuaACGG----ACUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 55345 | 0.68 | 0.890089 |
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Target: 5'- -gCGGUGGCGAUGcCGaacGCCUGAGc -3' miRNA: 3'- gaGCUACUGCUGCuGUaa-CGGACUCu -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 16676 | 0.68 | 0.904232 |
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Target: 5'- cCUCGAUGugGuCGGCGau-CCUGGGc -3' miRNA: 3'- -GAGCUACugCuGCUGUaacGGACUCu -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 43241 | 0.67 | 0.923366 |
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Target: 5'- -gCGAgcUGACGAUGccgcccGCGccgggUGCCUGAGAg -3' miRNA: 3'- gaGCU--ACUGCUGC------UGUa----ACGGACUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 61027 | 0.67 | 0.934713 |
|
Target: 5'- aCUCGGUGGCGGCGAgGUcGaCCgacGGGc -3' miRNA: 3'- -GAGCUACUGCUGCUgUAaC-GGa--CUCu -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 68691 | 0.66 | 0.939962 |
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Target: 5'- uCUCGAUGAUcgaGGCgGGCAgcgcgucggUGCCcGAGAa -3' miRNA: 3'- -GAGCUACUG---CUG-CUGUa--------ACGGaCUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 31666 | 0.66 | 0.939962 |
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Target: 5'- gCUUGAccuCGACGGgGUUGCCcGAGGc -3' miRNA: 3'- -GAGCUacuGCUGCUgUAACGGaCUCU- -5' |
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| 23502 | 3' | -50.8 | NC_005259.1 | + | 7741 | 0.66 | 0.949624 |
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Target: 5'- cCUCGAgGACG-CGGuCAccGUCUGAGAc -3' miRNA: 3'- -GAGCUaCUGCuGCU-GUaaCGGACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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